About Enhancer
| Enhancer ID: | E_02_0952 |
| Species: | human |
| Position : | chr5:44385749-44386845   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 22303449 |
| Enhancer experiment: | ChIP,EMSA |
| Enhancer experiment description: | In conjunction, we identified a novel enhancer containing a highly conserved ISL1 consensus binding site within the FGF10 first intron. ChIP and EMSA demonstrated its direct occupation by ISL1. Transcription mediated by ISL1 from this FGF10 intronic element was enhanced by the presence of GATA4 and TBX20 cardiac transcription factors. Finally, transgenic mice confirmed that endogenous factors bound the human FGF10 intronic enhancer to drive reporter expression in the developing cardiac outflow tract. |
About Target gene
| Target gene : | FGF10(FGF10) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP,EMSA |
| Target gene experiment description: | Using chromatin immunoprecipitation (ChIP) of microdissected embryonic human hearts, we demonstrated that at Carnegie stages 1415 (3336 dpf), ISL1 bound to and enriched a 327 bp FGF10-Int1 fragment (Fig. 2A). In contrast, ISL1 did not occupy FGF10-Pr1 or FGF10-Pr2. Acetylated histone H4 did bind both the ISL1 and FGF10 promoters at CS14-15, confirming that the chromatin around these two promoters is transcriptionally active in the human heart at these stages. |
About TF
| TF name : | ISL1(ISLET1,Isl-1)GATA4(ASD2,TACHD,TOF,VSD1)TBX20(ASD4) |
| TF experiment: | ChIP,EMSA |
| TF experiment description: | ChIP and EMSA demonstrated its direct occupation by ISL1. Transcription mediated by ISL1 from this FGF10 intronic element was enhanced by the presence of GATA4 and TBX20 cardiac transcription factors. |
About Function
| Enhancer function : | Endogenous factors bound the human FGF10 intronic enhancer to drive reporter expression in the developing cardiac outflow tract. |
| Enhancer function experiment: | ChIP,EMSA,Transgenic mice |
| Enhancer function experiment description: |
Finally, transgenic mice confirmed that endogenous factors bound the human FGF10 intronic enhancer to drive reporter expression in the developing cardiac outflow tract. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| ISL1 | Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP) | reactome | 13 |
| ISL1 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
| ISL1 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
| GATA4 | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| GATA4 | Notch-mediated HES/HEY network | pid | 48 |
| GATA4 | Physiological factors | reactome | 12 |
| GATA4 | Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP) | reactome | 13 |
| GATA4 | YAP1- and WWTR1 (TAZ)-stimulated gene expression | reactome | 29 |
| GATA4 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| GATA4 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
| GATA4 | Hs_Serotonin_Receptor_2_and_ELK-SRF-GATA4_signaling_WP732_80010 | wikipathways | 13 |
| TBX20 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene