About Enhancer
| Enhancer ID: | E_02_0955 |
| Species: | human |
| Position : | chr19:51354804-51355203   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 22212979 |
| Enhancer experiment: | qRT-PCR,ChIP |
| Enhancer experiment description: | Chromatin immunoprecipitation (ChIP) on the promoters and enhancers of prostate specific antigen (PSA) and transmembrane protease, serine(TMPRSS2) genes was performed.For PSA, in addition to the AREs located 131 and 150 bp from the tran_x0002_scription start site (TSS), an enhancer region 4 kb upstream of the TSS has been documented.For the TMPRSS2 gene, the AREs in the regulatory region are located 13.5 kb [27,38] and 700 bp up_x0002_stream of the TSS. |
About Target gene
| Target gene : | KLK3(APS,KLK2A1,PSA,hK3) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP-qPCR,ChIP |
| Target gene experiment description: | Chromatin immunoprecipitation(ChIP) on the promoters and enhancers of prostate specific antigen (PSA) and transmembrane protease, serine 2(TMPRSS2) genes was performed. qRT-PCR was used to measure the levels of PSA and TMPRSS2 transcripts.We performed ChIP-qPCR analyses on these selected genomic sites of PSA and TMPRSS2. |
About TF
| TF name : | AR(AIS8,DHTR,HUMARA,HYSP1,KD,NR3C4,SBMA,SMAX1,TFM,AR) |
| TF experiment: | ChIP-qPCR |
| TF experiment description: | At the 1 hr time point, AR loading onto the TMPRSS2 enhancer (Fig. 3A) was already significantly (P < 0.001, t-test) higher in LNCaP-ARhi cells than in the control cells. However, the TMPRSS2 promoter did not significantly recruit AR, at least in the 4 hr time (Fig. 3B).ChIP-qPCR was performed to assess the AR loading. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene