About Enhancer
| Enhancer ID: | E_02_0957 |
| Species: | human |
| Position : | chr5:134350424-134352424   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 21868451 |
| Enhancer experiment: | ChIP,Luciferase Reporter Assay,3C |
| Enhancer experiment description: | Genetic locus from the UCSC genome browser showing the natural 5 to 3 orientation of the PITX1 gene with the location of the identified ER binding regions, the proximal promoter and enhancer, and the location of the primers used in ChIP and chromosome conformation capture experiments. |
About Target gene
| Target gene : | PITX1(BFT,CCF,LBNBG,POTX,PTX1) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,Transfection |
| Target gene experiment description: | ER regulation of PITX1 involves interaction of the ER-binding distal enhancer with the promoter of the PITX1 gene via intrachromosomal looping.We next determined whether the PITX1 enhancer and promoter regions were estrogen responsive by cloning these regions from genomic DNA into luciferase reporters, and we conducted transient transfection assays. |
About TF
| TF name : | ESR1(ER,ESR,ESRA,ESTRR,Era,NR3A1) |
| TF experiment: | ChIP |
| TF experiment description: | Recruitment,monitored by ChIP,of ER and PITX1 to ER binding sites that contain a consensus PITX1 binding site in vehicle and 10 nM E2-treated 231ER cell at 45 min (panel D) or to ER binding sites that lack a consensus PITX1 binding site in vehicle and 10 nM E2-treated 231ER cell at 45 min. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene