About Enhancer

Enhancer ID: E_02_0961
Species: human
Position : chr12:7940709-7941994
Biosample name:
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: --
Pubmed ID:  21689639
Enhancer experiment: Luciferase Reporter Assay
Enhancer experiment description: Genome sequence analysis of the enhancer region of the human NANOG gene showed the presence of two conserved Tcf/Lef--binding motifs: CAAAGA at positions --1206 to --1190 (designated as Lef #1) and an inverted GTTTCA sequence at ?1020 to ?1004 (designated as Lef #2) (Fig. 1A). To investigate whether Tcf/Lef elements located in the enhancer region play a role in the regulation of NANOG gene transcription, we isolated a 1286--bp fragment of the enhancer region of the human NANOG gene and subcloned it into a firefly luciferase reporter vector, yielding pNanog--Luc(--1286/--1).

About Target gene

Target gene : NANOG(NANOG)
Strong evidence: --
Less strong evidence: Luciferase Reporter Assay
Target gene experiment description: To investigate whether Tcf/Lef elements located in the enhancer region play a role in the regulation of NANOG gene transcription, we isolated a 1286--bp fragment of the enhancer region of the human NANOG gene and subcloned it into a firefly luciferase reporter vector, yielding pNanog--Luc(--1286/--1).

About TF

TF name : LEF1(LEF-1,TCF10,TCF1ALPHA,TCF7L3)
TF experiment: ChIP
TF experiment description: ChIP experiments demonstrated that b-catenin and Lef1 bind to the Enhancer region of the NANOG gene. Our present findings would appear to indicate that the Tcf/Lef element in the Enhancer region of the Human NANOG gene functions in transcriptional activation of the Human NANOG gene.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:		 --

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
LEF1 Alzheimer disease-presenilin pathway panther 98
LEF1 Binding of TCF/LEF:CTNNB1 to target gene promoters reactome 7
LEF1 C-MYB transcription factor network pid 87
LEF1 Ca2+ pathway reactome 62
LEF1 Cadherin signaling pathway panther 147
LEF1 Canonical Wnt signaling pathway ( C. elegans endoderm induction Wnt signaling pathway Diagram ) inoh 56
LEF1 Canonical Wnt signaling pathway ( Canonical Wnt signaling pathway Diagram ) inoh 56
LEF1 Canonical Wnt signaling pathway ( Drosophila Wingless/Wnt signaling pathway Diagram ) inoh 56
LEF1 Canonical Wnt signaling pathway ( Mammalian Wnt signaling pathway Diagram ) inoh 56
LEF1 Canonical Wnt signaling pathway ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 56
LEF1 Deactivation of the beta-catenin transactivating complex reactome 42
LEF1 Formation of the beta-catenin:TCF transactivating complex reactome 88
LEF1 Integrin-linked kinase signaling pid 46
LEF1 Mammalian Wnt signaling pathway ( Mammalian Wnt signaling pathway Diagram ) inoh 49
LEF1 Notch netpath 76
LEF1 Regulation of nuclear beta catenin signaling and target gene transcription pid 80
LEF1 Repression of WNT target genes reactome 14
LEF1 Signaling with Wnt (Canonical) ( C. elegans endoderm induction Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Canonical) ( Canonical Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Canonical) ( Drosophila Wingless/Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Canonical) ( Mammalian Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Canonical) ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Mammal) ( Mammalian Wnt signaling pathway Diagram ) inoh 47
LEF1 Signaling with Wnt (Xenopus) ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 19
LEF1 Signaling without Wnt (Canonical) ( C. elegans endoderm induction Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Canonical) ( Canonical Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Canonical) ( Drosophila Wingless/Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Canonical) ( Mammalian Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Canonical) ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 8
LEF1 Signaling without Wnt (Mammal) ( Mammalian Wnt signaling pathway Diagram ) inoh 8
LEF1 TGF_beta_Receptor netpath 220
LEF1 Wnt netpath 118
LEF1 Wnt signaling pathway panther 250
LEF1 Wnt signaling pathway kegg 135
LEF1 Adherens junction kegg 70
LEF1 Melanogenesis kegg 100
LEF1 Pathways in cancer kegg 321
LEF1 Colorectal cancer kegg 63
LEF1 Endometrial cancer kegg 52
LEF1 Prostate cancer kegg 85
LEF1 Acute myeloid leukemia kegg 53
LEF1 Arrhythmogenic right ventricular cardiomyopathy (ARVC) kegg 71
LEF1 Hs_Wnt_Signaling_Pathway_Netpath_WP363_78571 wikipathways 28
LEF1 Hs_Endoderm_Differentiation_WP2853_88152 wikipathways 62
LEF1 Hs_Mesodermal_Commitment_Pathway_WP2857_87780 wikipathways 47

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs