About Enhancer

Enhancer ID: E_02_0966
Species: human
Position : chr17:32565596-32566796
Biosample name:
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: --
Pubmed ID:  21551367
Enhancer experiment: Luciferase Reporter Assay,PCR,ChIP,EMSA,3C
Enhancer experiment description: Schematic of CCL2 locus showing regions that were tested in the reporter assays.

About Target gene

Target gene : CCL2(GDCF-2,HC11,HSMCR30,MCAF,MCP-1,MCP1,SCYA2,SMC-CF)
Strong evidence: 3C
Less strong evidence: PCR,qPCR
Target gene experiment description: The 16.5 ECR physically interacts with CCL2 proximal promoter.To detect such physical interactions we used the 3C assay. In the 3C assay, interaction between widely separated regulatory elements is detected.

About TF

TF name : NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50)SP1(SP1)CEBPB(C/EBP-beta,IL6DBP,NF-IL6,TCF5)
TF experiment: ChIP,EMSA
TF experiment description: The EMSA and the pulldown experiments demonstrated in vitro interaction of the 16.5 ECR with NF-kB and c/EBPb. We used ChIP assay to determine whether the 16.5 ECR can bind NF-kB and c/EBPb in vivo. Sheared chromatin that was obtained from U87MG cells (treated or not treated with TNF-a) was immmu_x0002_noprecipitated with Abs directed against p65 and p50 subunits of NF-kB and c/EBPb or with control Abs.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:		 --

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
NFKB1 Activation of NF-kappaB in B cells reactome 66
NFKB1 Alternative NF-kappaB pathway pid 6
NFKB1 amb2 Integrin signaling pid 32
NFKB1 Angiopoietin receptor Tie2-mediated signaling pid 50
NFKB1 Apoptosis signaling pathway panther 104
NFKB1 Atypical NF-kappaB pathway pid 14
NFKB1 BCR netpath 161
NFKB1 BCR signaling pathway pid 70
NFKB1 Canonical NF-kappaB pathway pid 24
NFKB1 CD209 (DC-SIGN) signaling reactome 21
NFKB1 CD40/CD40L signaling pid 36
NFKB1 Ceramide signaling pathway pid 49
NFKB1 CLEC7A (Dectin-1) signaling reactome 78
NFKB1 CLEC7A/inflammasome pathway reactome 6
NFKB1 DEx/H-box helicases activate type I IFN and inflammatory cytokines production reactome 13
NFKB1 Downstream TCR signaling reactome 101
NFKB1 EGFR1 netpath 475
NFKB1 EPO signaling pathway pid 33
NFKB1 Fc-epsilon receptor I signaling in mast cells pid 64
NFKB1 FCERI mediated NF-kB activation reactome 88
NFKB1 Glucocorticoid receptor regulatory network pid 85
NFKB1 HIV-1 Nef: Negative effector of Fas and TNF-alpha pid 33
NFKB1 IkBA variant leads to EDA-ID reactome 7
NFKB1 IKBKG deficiency causes anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID) (via TLR) reactome 8
NFKB1 IKK-NF-kappaB cascade ( B cell receptor signaling ) inoh 8
NFKB1 IKK-NF-kappaB cascade ( CD4 T cell receptor signaling (NF-kB cascade) ) inoh 8
NFKB1 IKK-NF-kappaB cascade ( CD4 T cell receptor signaling ) inoh 8
NFKB1 IKK-NFkB cascade ( TNFR1 signaling pathway ) inoh 11
NFKB1 IL1-mediated signaling events pid 34
NFKB1 IL1 netpath 69
NFKB1 IL12-mediated signaling events pid 62
NFKB1 IL2 netpath 81
NFKB1 IL2 signaling events mediated by PI3K pid 38
NFKB1 IL23-mediated signaling events pid 37
NFKB1 IL3 netpath 84
NFKB1 IL4 netpath 75
NFKB1 IL5 netpath 59
NFKB1 IL6 netpath 85
NFKB1 Interleukin-1 processing reactome 7
NFKB1 Intracellular Signalling Through Adenosine Receptor A2a and Adenosine smpdb 37
NFKB1 Intracellular Signalling Through Adenosine Receptor A2b and Adenosine smpdb 38
NFKB1 Leptin netpath 98
NFKB1 LPA receptor mediated events pid 65
NFKB1 MAP3K8 (TPL2)-dependent MAPK1/3 activation reactome 16
NFKB1 NF-kB is activated and signals survival reactome 13
NFKB1 NF-kB signaling ( Toll-like receptor signaling pathway (trough NF-kappaB) ) inoh 12
NFKB1 Notch netpath 76
NFKB1 Osteopontin-mediated events pid 32
NFKB1 Prolactin netpath 105
NFKB1 RANKL netpath 84
NFKB1 Regulated proteolysis of p75NTR reactome 10
NFKB1 Regulation of Telomerase pid 70
NFKB1 RIP-mediated NFkB activation via ZBP1 reactome 21
NFKB1 Senescence-Associated Secretory Phenotype (SASP) reactome 108
NFKB1 Signaling events mediated by HDAC Class I pid 89
NFKB1 T cell receptor signaling (PLC gamma, PKC, Ras and IKK-NF-kappaB cascade) ( CD4 T cell receptor signaling (NF-kB cascade) ) inoh 30
NFKB1 T cell receptor signaling pathway ( CD4 T cell receptor signaling ) inoh 33
NFKB1 TAK1 activates NFkB by phosphorylation and activation of IKKs complex reactome 26
NFKB1 TCR netpath 261
NFKB1 TGF_beta_Receptor netpath 220
NFKB1 TNF receptor signaling pathway pid 46
NFKB1 TNFalpha netpath 274
NFKB1 TRAF6 mediated NF-kB activation reactome 24
NFKB1 Transcriptional regulation of white adipocyte differentiation reactome 79
NFKB1 TSLP netpath 24
NFKB1 TWEAK netpath 35
NFKB1 MAPK signaling pathway kegg 264
NFKB1 Chemokine signaling pathway kegg 187
NFKB1 Apoptosis kegg 88
NFKB1 Toll-like receptor signaling pathway kegg 100
NFKB1 NOD-like receptor signaling pathway kegg 60
NFKB1 RIG-I-like receptor signaling pathway kegg 71
NFKB1 Cytosolic DNA-sensing pathway kegg 56
NFKB1 T cell receptor signaling pathway kegg 105
NFKB1 B cell receptor signaling pathway kegg 70
NFKB1 Neurotrophin signaling pathway kegg 126
NFKB1 Adipocytokine signaling pathway kegg 65
NFKB1 Epithelial cell signaling in Helicobacter pylori infection kegg 67
NFKB1 Shigellosis kegg 60
NFKB1 Leishmaniasis kegg 71
NFKB1 Chagas disease kegg 103
NFKB1 Toxoplasmosis kegg 131
NFKB1 Amoebiasis kegg 103
NFKB1 Hepatitis C kegg 134
NFKB1 Pathways in cancer kegg 321
NFKB1 Pancreatic cancer kegg 70
NFKB1 Prostate cancer kegg 85
NFKB1 Chronic myeloid leukemia kegg 69
NFKB1 Acute myeloid leukemia kegg 53
NFKB1 Small cell lung cancer kegg 83
NFKB1 Hs_Apoptosis-related_network_due_to_altered_Notch3_in_ovarian_cancer_WP2864_79278 wikipathways 51
NFKB1 Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 wikipathways 41
NFKB1 Hs_Factors_and_pathways_affecting_insulin-like_growth_factor_(IGF1)-Akt_signaling_WP3850_88165 wikipathways 19
NFKB1 Hs_Leptin_signaling_pathway_WP2034_89856 wikipathways 37
NFKB1 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
NFKB1 Hs_Apoptosis_Modulation_and_Signaling_WP1772_91293 wikipathways 71
NFKB1 Hs_Structural_Pathway_of_Interleukin_1_(IL-1)_WP2637_87707 wikipathways 34
NFKB1 Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44
NFKB1 Hs_TNF_related_weak_inducer_of_apoptosis_(TWEAK)_Signaling_Pathway_WP2036_89910 wikipathways 27
NFKB1 Hs_Apoptosis_Modulation_by_HSP70_WP384_67054 wikipathways 10
NFKB1 Hs_Glucocorticoid_&_Mineralcorticoid_Metabolism_WP237_78497 wikipathways 14
NFKB1 Hs_Allograft_Rejection_WP2328_90020 wikipathways 12
NFKB1 Hs_Oxidative_Damage_WP3941_90810 wikipathways 12
NFKB1 Hs_T-Cell_Receptor_and_Co-stimulatory_Signaling_WP2583_86147 wikipathways 11
NFKB1 Hs_MicroRNAs_in_cardiomyocyte_hypertrophy_WP1544_89794 wikipathways 29
NFKB1 Hs_TNF_alpha_Signaling_Pathway_WP231_89895 wikipathways 61
NFKB1 Hs_IL17_signaling_pathway_WP2112_89838 wikipathways 20
NFKB1 Hs_Estrogen_signaling_pathway_WP712_78491 wikipathways 13
NFKB1 Hs_Gene_regulatory_network_modelling_somitogenesis_WP2854_87527 wikipathways 12
NFKB1 Hs_TLR4_Signaling_and_Tolerance_WP3851_90114 wikipathways 8
NFKB1 Hs_IL1_and_megakaryocytes_in_obesity_WP2865_88662 wikipathways 5
NFKB1 Hs_NAD_metabolism,_sirtuins_and_aging_WP3630_88178 wikipathways 8
NFKB1 Hs_Prolactin_Signaling_Pathway_WP2037_90015 wikipathways 52
NFKB1 Hs_AGE-RAGE_pathway_WP2324_89798 wikipathways 27
NFKB1 Hs_IL-4_Signaling_Pathway_WP395_89828 wikipathways 41
NFKB1 Hs_Brain-Derived_Neurotrophic_Factor_(BDNF)_signaling_pathway_WP2380_89803 wikipathways 80
NFKB1 Hs_T-Cell_antigen_Receptor_(TCR)_Signaling_Pathway_WP69_89937 wikipathways 60
NFKB1 Hs_IL-1_signaling_pathway_WP195_89932 wikipathways 34
NFKB1 Hs_RANKL-RANK_(Receptor_activator_of_NFKB_(ligand))_Signaling_Pathway_WP2018_90016 wikipathways 26
NFKB1 Hs_B_Cell_Receptor_Signaling_Pathway_WP23_89900 wikipathways 71
SP1 Activation of gene expression by SREBF (SREBP) reactome 42
SP1 AndrogenReceptor netpath 167
SP1 AP-1 transcription factor network pid 71
SP1 C-MYB transcription factor network pid 87
SP1 Direct p53 effectors pid 141
SP1 E2F transcription factor network pid 77
SP1 EGFR1 netpath 475
SP1 FOXA1 transcription factor network pid 45
SP1 FOXA2 and FOXA3 transcription factor networks pid 45
SP1 FOXM1 transcription factor network pid 43
SP1 HIF-1-alpha transcription factor network pid 67
SP1 HIF-2-alpha transcription factor network pid 34
SP1 Huntington disease panther 113
SP1 IL2 signaling events mediated by STAT5 pid 30
SP1 IL4-mediated signaling events pid 66
SP1 Leptin netpath 98
SP1 Oncogene Induced Senescence reactome 30
SP1 p73 transcription factor network pid 80
SP1 PPARA activates gene expression reactome 113
SP1 Regulation of nuclear SMAD2/3 signaling pid 82
SP1 Regulation of Telomerase pid 70
SP1 RNA polymerase II transcribes snRNA genes reactome 70
SP1 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription reactome 32
SP1 TGF_beta_Receptor netpath 220
SP1 Validated targets of C-MYC transcriptional repression pid 63
SP1 Validated transcriptional targets of AP1 family members Fra1 and Fra2 pid 37
SP1 Validated transcriptional targets of TAp63 isoforms pid 54
SP1 TGF-beta signaling pathway kegg 82
SP1 Huntington's disease kegg 182
SP1 Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 wikipathways 41
SP1 Hs_Leptin_signaling_pathway_WP2034_89856 wikipathways 37
SP1 Hs_Interleukin-11_Signaling_Pathway_WP2332_79525 wikipathways 17
SP1 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
SP1 Hs_Estrogen_signaling_pathway_WP712_78491 wikipathways 13
SP1 Hs_Sudden_Infant_Death_Syndrome_(SIDS)_Susceptibility_Pathways_WP706_86078 wikipathways 33
SP1 Hs_TGF-beta_Signaling_Pathway_WP366_90028 wikipathways 67
SP1 Hs_AGE-RAGE_pathway_WP2324_89798 wikipathways 27
SP1 Hs_Androgen_receptor_signaling_pathway_WP138_79958 wikipathways 27
SP1 Hs_EGF-EGFR_Signaling_Pathway_WP437_79266 wikipathways 107
CEBPB C-MYB transcription factor network pid 87
CEBPB EGFR1 netpath 475
CEBPB FOXA1 transcription factor network pid 45
CEBPB FOXA2 and FOXA3 transcription factor networks pid 45
CEBPB IFN-gamma pathway pid 43
CEBPB IL3-mediated signaling events pid 27
CEBPB IL4-mediated signaling events pid 66
CEBPB IL4 netpath 75
CEBPB IL6-mediated signaling events pid 48
CEBPB IL6 netpath 85
CEBPB RANKL netpath 84
CEBPB Regulation of nuclear SMAD2/3 signaling pid 82
CEBPB Regulation of retinoblastoma protein pid 67
CEBPB Senescence-Associated Secretory Phenotype (SASP) reactome 108
CEBPB Signaling mediated by p38-alpha and p38-beta pid 35
CEBPB TCR netpath 261
CEBPB Transcriptional regulation of white adipocyte differentiation reactome 79
CEBPB Validated nuclear estrogen receptor alpha network pid 65
CEBPB Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193 wikipathways 62
CEBPB Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 wikipathways 44
CEBPB Hs_Transcriptional_activation_by_NRF2_WP3_79527 wikipathways 6
CEBPB Hs_Lung_fibrosis_WP3624_91475 wikipathways 23
CEBPB Hs_IL17_signaling_pathway_WP2112_89838 wikipathways 20

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs