About Enhancer
| Enhancer ID: | E_02_0967 |
| Species: | human |
| Position : | chr18:61099404-61099740   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 21549805 |
| Enhancer experiment: | Luciferase Reporter Assay,EMSA,ChIP |
| Enhancer experiment description: | luciferase expression was driven by FIRE sequences, and tested these constructs for their responsiveness to PAX5 expression in the RAW264 macrophage cell line and the 38B9 B-cell line using transient transfection assays.When testing the binding of PAX5 across Csf1r by ChIPassays in primary pro-B cells, we noticed that PAX5 did not just bind to the promoter, but also associated with the intronic enhancer (FIRE).This observation was confirmed by EMSA experiments, demonstrating that none of the DNA sequences present on FIRE were capable of competing for the binding of PAX5 to DNA containing a PAX5 consensus sequence. |
About Target gene
| Target gene : | Csf1r(BANDDOS,C-FMS,CD115,CSF-1R,CSFR,FIM2,FMS,HDLS,M-CSF-R) |
| Strong evidence: | -- |
| Less strong evidence: | Immunoprecipitation,Transfection,ChIP |
| Target gene experiment description: | We performed PAX5-specific chromatin immunoprecipitation analyses across the Csfr1 locus. We investigated the role of PAX5 in regulating Csf1r sense and antisense promoter activity by transient transfections and by employing a Pax5/ proLBcell line expressing an inducible PAX5 protein. When testing the binding of PAX5 across Csf1r by ChIPassays in primary pro-B cells, we noticed that PAX5 did not just bind to the promoter, but also associated with the intronic enhancer (FIRE). |
About TF
| TF name : | Pax5(ALL3,BSAP) |
| TF experiment: | ChIP,EMSA |
| TF experiment description: | PAX5 binds to the intronic enhancer of Csf1r (FIRE).When testing the binding of PAX5 across Csf1r by ChIP assays in primary pro-B cells, we noticed that PAX5 did not just bind to the promoter, but also associated with the intronic Enhancer (FIRE).This observation was confirmed by EMSA experiments. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Pax5 | C-MYB transcription factor network | pid | 87 |
| Pax5 | ID | netpath | 38 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene