About Enhancer
| Enhancer ID: | E_02_0970 |
| Species: | human |
| Position : | chr5:115358083-115360083   |
| Biosample name: | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 21486496 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP-seq |
| Enhancer experiment description: | The deleted BAC constructs were introduced into ESCs, and stable transformants were established for each BAC reporter construct. In a similar manner to the RA treatment and Luciferase reporter assay conducted with EBs (shown in Figure ?Figure2A),the Msi1 transcriptional activities were quantified using the deletion-containing reporters D1-D5 and the full-length BAC reporter D0. These results suggested that the 10-kb region from 55 kb to 65 kb upstream of the TSS might contain Msi1 transcriptional enhancers, and we named this region the 'upstream 10-kb enhancer.' |
About Target gene
| Target gene : | Msi1(Msi1h,Musahi1,m-Msi-1) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay |
| Target gene experiment description: | We then used homologous recombination techniques to insert the ffLuc reporter gene into the Msi1 translational initiation site of the RP24-132L16 BAC (Figure1A).The ffLuc reporter gene encodes a fusion protein of the fluorescent protein Venus and firefly Luciferase.This reporter gene both visualized Msi1 transcriptional activity in vivo, and allowed us to use Luciferase bioluminescence to quantify the level of transcriptional activity. |
About TF
| TF name : | Qsox1(1300003H02Rik,QSOX,Qscn6,SOx,b2b2673Clo)Tfap2a(AP-2,AP2alpha,Ap-2 (a),Ap2,Ap2tf,Tcfap2a) |
| TF experiment: | Luciferase Reporter Assay |
| TF experiment description: | Through these investigations, we have identified a new Msi1 transcription Enhancer element in NS/PCs. This region is located in the 595-bp region within the sixth intron of the Msi1 gene, and contains SOX- and AP-2-binding sites. |
About Function
| Enhancer function : | The intronic Enhancer can transactivate Msi1 gene expression with cell-type specificity markedly similar to the endogenous Msi1 expression patterns. |
| Enhancer function experiment: | Luciferase Reporter Assay |
| Enhancer function experiment description: |
Furthermore, when the region after the transcription site (exons and introns coding region) was deleted, the Msi1 transcriptional activity diminished even in the presence of theupstream 10-kb Enhancer (Figure 3C). These results indicated two regions responsible for Msi2 transcription. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Qsox1 | Platelet degranulation | reactome | 107 |
| Tfap2a | Caspase Cascade in Apoptosis | pid | 57 |
| Tfap2a | Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 | wikipathways | 41 |
| Tfap2a | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
| Tfap2a | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
| Tfap2a | Hs_Hypothetical_Craniofacial_Development_Pathway_WP3655_88473 | wikipathways | 5 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene