About Enhancer

Enhancer ID: E_02_0974
Species: human
Position : chr11:32403489-32405489
Biosample name:
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: --
Pubmed ID:  21378152
Enhancer experiment: qRT-PCR,Luciferase Reporter Assay
Enhancer experiment description: We tested the effect of TGF-beta1 on a human WT1 enhancer located ?4.3 kb upstream of the transcription start site using a reporter vector WTA.

About Target gene

Target gene : WT1(AWT1,GUD,NPHS4,WAGR,WIT-2,WT33)
Strong evidence: --
Less strong evidence: qRT-PCR,Luciferase Reporter Assay
Target gene experiment description: TGF-beta1 did not alter luciferase activity of the reporter construct for a human WT1 promoter but reduced that for a human WT1 5? enhancer construct, suggesting that TGF-beta1 may regulate WT1 expression by altering the 5? enhancer activity.

About TF

TF name : SMAD4(DPC4,JIP,MADH4,MYHRS)
TF experiment: Luciferase Reporter Assay
TF experiment description: The luciferase activity of p3TP-lux, a reporter construct for Smadbinding element, was determined after incubation with 5 ng/mL TGFbeta1 for 24 h. TGF-beta1 increased the luciferase activity by ~12-fold in the presence of the NTC shRNA, while this increase was largely prevented by Sh-Smad4. The luciferase activity of p3TP-lux was normalized to beta-galactosidase activity of pCMV-beta-gal and shown as relative values to that of pGL2 basic.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:		 --

About SNP

SNP ID: --

Upstream Pathway Annotation of TF

GeneName Pathway Name Source Gene Number
SMAD4 ALK1 signaling events pid 26
SMAD4 ALK2 signaling events pid 11
SMAD4 BMP receptor signaling pid 42
SMAD4 BMP signaling pathway ( TGF-beta_BMP Diagram(MolecularVariation) ) inoh 34
SMAD4 BMP Signalling Pathway humancyc 5
SMAD4 BMP2 signaling pathway(through Smad) ( TGF-beta_BMP Diagram(MolecularVariation) ) inoh 13
SMAD4 Downregulation of SMAD2/3:SMAD4 transcriptional activity reactome 23
SMAD4 Gene expression of smad6/7 by R-smad:smad4 ( TGF-beta_super_family_signaling_pathway(canonical) ) inoh 8
SMAD4 Gene expression of smad7 by R-smad:smad4 ( TGF-beta_super_family_signaling_pathway(canonical) ) inoh 7
SMAD4 HIF-1-alpha transcription factor network pid 67
SMAD4 LIF signaling pathway ( LIF signaling(JAK1 JAK2 STAT3) ) inoh 10
SMAD4 LKB1 signaling events pid 43
SMAD4 Negative regulation of (transcription by R-smad:smad4) in TGF beta super family signaling pathway ( TGF-beta_super_family_signaling_pathway(canonical) ) inoh 21
SMAD4 Regulation of cytoplasmic and nuclear SMAD2/3 signaling pid 20
SMAD4 Regulation of nuclear SMAD2/3 signaling pid 82
SMAD4 Signaling by Activin reactome 11
SMAD4 Signaling by BMP reactome 23
SMAD4 Signaling by NODAL reactome 19
SMAD4 Signaling with Wnt (Canonical) ( C. elegans endoderm induction Wnt signaling pathway Diagram ) inoh 47
SMAD4 Signaling with Wnt (Canonical) ( Canonical Wnt signaling pathway Diagram ) inoh 47
SMAD4 Signaling with Wnt (Canonical) ( Drosophila Wingless/Wnt signaling pathway Diagram ) inoh 47
SMAD4 Signaling with Wnt (Canonical) ( Mammalian Wnt signaling pathway Diagram ) inoh 47
SMAD4 Signaling with Wnt (Canonical) ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 47
SMAD4 Signaling with Wnt (Mammal) ( Mammalian Wnt signaling pathway Diagram ) inoh 47
SMAD4 Signaling with Wnt (Xenopus) ( Xenopus axis formation Wnt signaling pathway Diagram ) inoh 19
SMAD4 SMAD2/3 MH2 Domain Mutants in Cancer reactome 7
SMAD4 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription reactome 32
SMAD4 TGF-beta receptor signaling activates SMADs reactome 12
SMAD4 TGF-beta receptor signaling pid 53
SMAD4 TGF-beta signaling pathway panther 88
SMAD4 TGF-beta_super_family_signaling_pathway(canonical) ( TGF-beta_BMP Diagram(MolecularVariation) ) inoh 51
SMAD4 TGF_beta_Receptor netpath 220
SMAD4 Transcriptional regulation of pluripotent stem cells reactome 28
SMAD4 Validated nuclear estrogen receptor alpha network pid 65
SMAD4 Validated targets of C-MYC transcriptional activation pid 80
SMAD4 Validated targets of C-MYC transcriptional repression pid 63
SMAD4 Wnt signaling pathway panther 250
SMAD4 Cell cycle kegg 124
SMAD4 Wnt signaling pathway kegg 135
SMAD4 TGF-beta signaling pathway kegg 82
SMAD4 Adherens junction kegg 70
SMAD4 Pathways in cancer kegg 321
SMAD4 Colorectal cancer kegg 63
SMAD4 Pancreatic cancer kegg 70
SMAD4 Chronic myeloid leukemia kegg 69
SMAD4 Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 wikipathways 122
SMAD4 Hs_Endoderm_Differentiation_WP2853_88152 wikipathways 62
SMAD4 Hs_Heart_Development_WP1591_90186 wikipathways 28
SMAD4 Hs_Mesodermal_Commitment_Pathway_WP2857_87780 wikipathways 47
SMAD4 Hs_Ectoderm_Differentiation_WP2858_89329 wikipathways 56
SMAD4 Hs_TGF-beta_Signaling_Pathway_WP366_90028 wikipathways 67

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer

Enhancer associated SNPs