About Enhancer
| Enhancer ID: | E_02_0976 |
| Species: | mouse |
| Position : | chr3:34563695-34565695   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 21331042 |
| Enhancer experiment: | EMSA,Immunostaining,Transgenic mice |
| Enhancer experiment description: | Our earlier studies have indicated that among a number of enhancers regulating Sox2, enhancer N1 is responsible for Sox2 activation in the caudally extendin neural plate5,6 (Fig.1a-c; Supplementary Fig.1). |
About Target gene
| Target gene : | Sox2(ANOP3,MCOPS3) |
| Strong evidence: | -- |
| Less strong evidence: | EMSA,Immunostaining |
| Target gene experiment description: | Finally, we asked whether the suppression of Sox2 enhancer N1 activation by Tbx6 involves direct interaction of the Tbx6 protein with the enhancer N1 DNA sequence. Various overlapping fragments of the N1 sequence were tested for Tbx6 binding using electrophoretic mobility shift assays (EMSA). Exogenous Sox2-HA was successfully expressed in the caudal-most mesodermal compartment (Fig.4b), as indicated by HA-tag immunostaining and the mesodermal Sox2 immunostaining (Fig.4c-e). This result demonstrates that Sox2 expression is sufficient for the initiation of neural tube development from the primitive paraxial mesoderm, even in wild type embryos. |
About TF
| TF name : | Tbx6(rv) |
| TF experiment: | EMSA |
| TF experiment description: | Various overlapping fragments of the N1 sequence were tested for Tbx6 binding using electrophoretic mobility shift assays (EMSA). |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Tbx6 | Hs_Gene_regulatory_network_modelling_somitogenesis_WP2854_87527 | wikipathways | 12 |
| Tbx6 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene