About Enhancer
| Enhancer ID: | E_02_0979 |
| Species: | human |
| Position : | chr1:23881789-23881796   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 21056086 |
| Enhancer experiment: | qRT-PCR,Luciferase Reporter Assay,ChIP |
| Enhancer experiment description: | To determine if these base pairs also play a role in BMP2induction of the human ID3 promoter, we introduced mutations comparable to Mut4 and Mut5 in the murine Id3 promoter into the minimal hID3 promoter--reporter construct (?653/+402 ID3--luc) (Fig. 6(B)). |
About Target gene
| Target gene : | ID3(HEIR-1,bHLHb25),ID3(HEIR-1,bHLHb25) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP |
| Target gene experiment description: | We identified the same sequence in the murine gene (?3283/?3276) (Fig. S8) and observed through ChIP analysis that BMP2 stimulated recruitment of SMAD1 to this region of the endogenous Id3 gene in L?T2 cells;We identified the same sequence in the murine gene (?3283/?3276) (Fig. S8) and observed through ChIP analysis that BMP2 stimulated recruitment of SMAD1 to this region of the endogenous Id3 gene in L?T2 cells |
About TF
| TF name : | BMP2(BDA2A,SSFSC,BMP2)BMP2(BDA2A,SSFSC,BMP2) |
| TF experiment: | ChIP |
| TF experiment description: | We identified the same sequence in the murine gene (?3283/?3276) (Fig. S8) and observed through ChIP analysis that BMP2 stimulated recruitment of SMAD1 to this region of the endogenous Id3 gene in L?T2 cells (Fig. S7A). Muta_x0002_tion of the distal BRE in the longer murine Id3 reporter greatly reduced, but did not abolish, BMP2 induction.;We identified the same sequence in the murine gene (?3283/?3276) (Fig. S8) and observed through ChIP analysis that BMP2 stimulated recruitment of SMAD1 to this region of the endogenous Id3 gene in L?T2 cells (Fig. S7A). Muta_x0002_tion of the distal BRE in the longer murine Id3 reporter greatly reduced, but did not abolish, BMP2 induction. |
About Function
| Enhancer function : | A more distal Enhancer was shown to mediate BMP4-induction of the human ID3 gene in ovarian cancer cells. |
| Enhancer function experiment: | ChIP |
| Enhancer function experiment description: |
We identified the same sequence in the murine gene (?3283/?3276) and observed through ChIP analysis that BMP2 stimulated recruitment of SMAD1 to this region of the endogenous Id3 gene in LT2 cells. Mutation of the distal BRE in the longer murine Id3 reporter greatly reduced, but did not abolish, BMP2 induction. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene