About Enhancer
| Enhancer ID: | E_02_0984 |
| Species: | mouse |
| Position : | chr3:48598405-48598558   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | Myocardial ischemia |
| Pubmed ID: | 19708858 |
| Enhancer experiment: | Luciferase Reporter Assay,RT-PCR |
| Enhancer experiment description: | This activation was conserved in constructs with the 3' FLR fragment placed upstream of the luciferase gene, indicating an enhancer function for HRE1.To test their role as enhancers, we cloned several fragments of the 3 'FLR of the hUcn2 gene downstream of the firefly luciferase gene in (a) the pGL3 ?746 bp vector containing the functional hUcn2 promoter, and (b) the pGL3control vector containing an internal SV40 promoter (Figure 2A). |
About Target gene
| Target gene : | UCN2(SRP,UCN-II,UCNI,UR,URP) |
| Strong evidence: | -- |
| Less strong evidence: | Luciferase Reporter Assay,Transfection |
| Target gene experiment description: | To test their role as enhancers, we cloned several fragments of the 3 ' FLR of the hUcn2 gene downstream of the firefly luciferase gene in (a) the pGL3 ?746 bp vector containing the functional hUcn2 promoter, and (b) the pGL3control vector containing an internal SV40 promoter (Figure 2A). TE--671 cells were transfected with the different constructs and exposed to normoxia or hypoxia for 12 h. Hypoxia significantly increased luciferase expression, specifically in the constructs containing the fragment 3 'FLR3 (Figure 2A). |
About TF
| TF name : | HIF1A(HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78) |
| TF experiment: | ELISA |
| TF experiment description: | To prove that HIF1? binds to HRE1 and HRE2 in the 3_x0002_FLR of the hUcn2 gene, we performed HIF1? binding and competition ELISA assays. After 12 h of hypoxia, binding of HIF1? to the EPO HRE was stronger than under normoxic conditions. |
About Function
| Enhancer function : | Hypoxia induces hUcn2 expression via a specific HRE in the 3 FLR of the hUcn2 gene, which interacts with the transcription factor HIF1?. |
| Enhancer function experiment: | Luciferase Reporter Assay |
| Enhancer function experiment description: |
Similar to the results obtained with constructs that contained the 3' FLR3 variants downstream of the luciferase gene, the hypoxia- and CPX-induced luciferase activation was strongest in the 3' FLR3 containing both wild-type HRE1 and HRE2. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene