About Enhancer
| Enhancer ID: | E_02_0985 |
| Species: | human |
| Position : | chr16:67519201-67519833   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 19285986 |
| Enhancer experiment: | Transgenic mice,Luciferase Reporter Assay |
| Enhancer experiment description: | Schematic representation of the genomic region encompassing the AgRP and the ATPase locus. Expression constructs for regions A, B, C, and D were directionally cloned into pGL3-basic luciferase vector.Regions C and D were evaluated in the same fashion. Region D (but not region C) contained enhancer elements in both neuronal and somatic cell types (Fig. 1a). Region D was therefore divided into three overlapping sub-regions: Di(-18/+312)(+2727/+3359), Dii(-18/+312) (+3341/+3941), and Diii(-18/+312)(+3943/+4575). Sub-regions Di and Diii (but not Dii) had enhancer properties for the AgRP basal promoter in both h295R and N38 cell lines (Fig. 1b).Fig. 1. In vitro determination of enhancer elements |
About Target gene
| Target gene : | AGRP(AGRT,ART,ASIP2) |
| Strong evidence: | -- |
| Less strong evidence: | Transgenic mice,Luciferase Reporter Assay |
| Target gene experiment description: | Region D (but not C) increased activity of the human AgRP promoter, equally in the human adrenocortical NCI-h295R and the mouse clonal hypothalamic N38 cell lines. |
About TF
| TF name : | -- |
| TF experiment: | -- |
| TF experiment description: | -- |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene