About Enhancer
| Enhancer ID: | E_02_0988 |
| Species: | mouse |
| Position : | chr7:126406352-126406616   |
| Biosample name: | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer |
| Disease: | -- |
| Pubmed ID: | 18552207 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP |
| Enhancer experiment description: | Transient transfection assays in different mouse cell lines. A mouse Cd19 promoter reporter construct (_x005f_x005f_x005f_x005f_x005f_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x005f_x005F_x0007_7 to _x005F_x0007_200 bp; e) or a construct containing the _x0007_2 kb DHS (_x0007_1832 to _x0007_2096 bp) combined with the Cd19 promoter (f) was transiently transfected into various cell lines. The relative activity was determined as the luciferase activity of each construct over control vector pXPG. Data represent the mean of 2 to 4 experiments performed in triplicate |
About Target gene
| Target gene : | Cd19(B4,CVID3) |
| Strong evidence: | -- |
| Less strong evidence: | ChIP |
| Target gene experiment description: | As previously reported in human cells, the promoter was active in all analyzed cell types (Figure 1C white bars). In contrast, the _x005F_x0007_2 kb DHS had enhancer activity only in B cells (Figure 1C black bars), providing evidence that this enhancer is involved in the regulation of tissue specific expression of Cd19. |
About TF
| TF name : | Tcf3(A1,AA408400,ALF2,AW209082,E12,E12/E47,E2A,E47,KA1,ME2,Pan1,Pan2,TCF-3,Tcfe2a,VDIR,bHLHb21)Ebf1(COE1,EBF,O/E-1,OLF1)Pax5(ALL3,BSAP) |
| TF experiment: | ChIP,DMS Footprinting Assay |
| TF experiment description: | We performed an in vivo DMS footprinting assay to answer the question of which transcription factors bind to the Cd19 promoter and enhancer.The specific binding of these proteins was confirmed by ChIP analysis, demonstrating that EBF and PAX5 bound to the enhancer in B cells and that E2A bound in B and T cells. |
About Function
| Enhancer function : | Enhancer is involved in the regulation of tissue specific expression of Cd19 |
| Enhancer function experiment: | Transfection |
| Enhancer function experiment description: |
As previously reported in human cells, the promoter was active in all analyzed cell types (Figure 1C white bars). In contrast, the -2 kb DHS had Enhancer activity only in B cells (Figure 1C black bars), providing evidence that this Enhancer is involved in the regulation of tissue specific expression of Cd19. |
About SNP
| SNP ID: | -- |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Tcf3 | CDO in myogenesis | reactome | 29 |
| Tcf3 | ID | netpath | 38 |
| Tcf3 | Notch-mediated HES/HEY network | pid | 48 |
| Tcf3 | Notch | netpath | 76 |
| Tcf3 | p38 signaling mediated by MAPKAP kinases | pid | 21 |
| Tcf3 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Tcf3 | Wnt | netpath | 118 |
| Tcf3 | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
| Ebf1 | Transcriptional regulation of white adipocyte differentiation | reactome | 79 |
| Ebf1 | Hs_Tryptophan_metabolism_WP465_85055 | wikipathways | 45 |
| Pax5 | C-MYB transcription factor network | pid | 87 |
| Pax5 | ID | netpath | 38 |
Enhancer associated network
The number on yellow line represents the distance between enhancer and
target gene