About Enhancer
| Enhancer ID: | E_00032 |
| Species: | Homo sapiens |
| Position : | chr6:174249537-174250490   |
| Year: | 2023 |
| Title: | Genome-wide CRISPR activation screening in senescent cells reveals SOX5 as a driver and therapeutic target of rejuvenation |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | Osteoarthritis |
| PMID: | 37832549 |
| MONDO: | MONDO:0001623 |
| Tissue: | Bone marrow |
| Tissue Ontology ID: | UBERON:0002371 |
| Cell Source: | WT hMPC |
| CVCL ID: | |
| Cell Type: | Human mesenchymal precursor cell |
| Cell Ontology ID: | CL:0000128 |
| Experiment Type: | Luciferase reporter assay, ChIP-qPCR, Hi-C chromatin interaction validation |
| High Throughput Method: | ATAC-seq, H3K27ac ChIP-seq, H3K4me1 ChIP-seq, SOX5 ChIP-seq, RNA-seq, Hi-C, single-cell multiome |
| Low Throughput Method: | dual-luciferase reporter detection, ChIP-qPCR, lentiviral overexpression, siRNA knockdown, CRISPRa perturbation, RT-qPCR, western blot |
About Target Gene
| Target gene : | HMGB2 |
About TF
| TF name : | SOX5 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| SOX5 | Hs_Endochondral_Ossification_WP474_87977 | wikipathways | 43 |
| SOX5 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
3 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_00029 | chr6:174249537-174250490 | Bone marrow | Human mesenchymal precursor cell | Cellular senescence |
| E_00030 | chr6:174249537-174250490 | Bone marrow | Human mesenchymal precursor cell | Cellular senescence |
| E_00031 | chr6:174249537-174250490 | Bone marrow | Human mesenchymal precursor cell | Osteoarthritis |
Expression of Target Genes for the Enhancer