About Enhancer
| Enhancer ID: | E_00049 |
| Species: | Homo sapiens |
| Position : | chr16:53800454-53801454   |
| Year: | 2015 |
| Title: | FTO Obesity Variant Circuitry and Adipocyte Browning in Humans. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | obesity |
| PMID: | 26287746 |
| MONDO: | MONDO:0011122 |
| Tissue: | subcutaneous adipose tissue |
| Tissue Ontology ID: | UBERON:0002190 |
| Cell Source: | SGBS |
| CVCL ID: | CVCL_GS28 |
| Cell Type: | preadipocyte |
| Cell Ontology ID: | CL:0002334 |
| Experiment Type: | Enhancer activity assay; endogenous allele editing; TF-binding assay; target-gene perturbation; Endogenous enhancer perturbation; Disease-variant functional analysis |
| High Throughput Method: | Roadmap epigenomic chromatin-state analysis; long-range chromatin interaction analysis; eQTL/trans-eQTL analysis; gene-expression profiling |
| Low Throughput Method: | Luciferase reporter assay; CRISPR-Cas9 single-nucleotide editing; EMSA; ARID5B knockdown/overexpression; IRX5 knockdown/overexpression; RT-qPCR; oxygen-consumption and thermogenesis assays; CRISPR-Cas9 endogenous deletion/editing; Allele-specific reporter assay |
About Target Gene
| Target gene : | IRX5 |
About TF
| TF name : | ARID5B |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| ARID5B | HDMs demethylate histones | reactome | 49 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
3 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_00048 | chr16:53800454-53801454 | subcutaneous adipose tissue | preadipocyte | obesity |
| E_00425 | chr16:53800454-53801454 | subcutaneous adipose tissue | subcutaneous preadipocyte | obesity |
| E_00426 | chr16:53800454-53801454 | subcutaneous adipose tissue | subcutaneous preadipocyte | obesity |
Expression of Target Genes for the Enhancer