About Enhancer
| Enhancer ID: | E_00096 |
| Species: | Homo sapiens |
| Position : | chr3:27713393-27715978   |
| Year: | 2023 |
| Title: | Dynamic network-guided CRISPRi screen identifies CTCF-loop-constrained nonlinear enhancer gene regulatory activity during cell state transitions |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| Disease Name: | -- |
| PMID: | 37488417 |
| MONDO: | |
| Tissue: | Definitive endoderm |
| Tissue Ontology ID: | UBERON:0005425 |
| Cell Source: | HUES8 |
| CVCL ID: | CVCL_B207 |
| Cell Type: | human embryonic stem cell |
| Cell Ontology ID: | CL:0000335 |
| Experiment Type: | dCas9-KRAB CRISPRi loss-of-function, Hi-C chromatin contact validation |
| High Throughput Method: | ATAC-seq, H3K27ac ChIP-seq, RNA-seq, scRNA-seq, pooled CRISPRi screening |
| Low Throughput Method: | RT-qPCR, FACS flow cytometry, lentiviral transduction, single enhancer clonal CRISPR-Cas9 deletion |
About Target Gene
| Target gene : | EOMES |
About TF
| TF name : | EOMES |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| EOMES | Downstream signaling in naïve CD8+ T cells | pid | 69 |
| EOMES | IL12-mediated signaling events | pid | 62 |
| EOMES | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01865 | chr3:27713390-27715985 | Inner cell mass | embryonic stem cell | -- |
Expression of Target Genes for the Enhancer