About Enhancer
| Enhancer ID: | E_00098 |
| Species: | Homo sapiens |
| Position : | chr18:22026135-22027396   |
| Year: | 2023 |
| Title: | Dynamic network-guided CRISPRi screen identifies CTCF-loop-constrained nonlinear enhancer gene regulatory activity during cell state transitions |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| Disease Name: | -- |
| PMID: | 37488417 |
| MONDO: | |
| Tissue: | Definitive endoderm |
| Tissue Ontology ID: | UBERON:0005425 |
| Cell Source: | HUES8 |
| CVCL ID: | CVCL_B207 |
| Cell Type: | human embryonic stem cell |
| Cell Ontology ID: | CL:0000335 |
| Experiment Type: | dCas9-KRAB CRISPRi loss-of-function, CRISPR-Cas9 clonal deletion, Hi-C chromatin contact validation |
| High Throughput Method: | ATAC-seq, H3K27ac ChIP-seq, RNA-seq, scRNA-seq, pooled CRISPRi screening |
| Low Throughput Method: | RT-qPCR, FACS flow cytometry, lentiviral transduction, dual clonal enhancer knockout generation |
About Target Gene
| Target gene : | GATA6 |
About TF
| TF name : | GATA6 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| GATA6 | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| GATA6 | Notch-mediated HES/HEY network | pid | 48 |
| GATA6 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| GATA6 | Surfactant metabolism | reactome | 30 |
| GATA6 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01862 | chr18:22026154-22027330 | Inner cell mass | embryonic stem cell | -- |
Expression of Target Genes for the Enhancer