About Enhancer
| Enhancer ID: | E_00137 |
| Species: | Homo sapiens |
| Position : | chr8:128900000-128900620   |
| Year: | 2024 |
| Title: | Selective Enhancer Gain of Function Deregulates MYC Expression in Multiple Myeloma |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | Multiple myeloma |
| PMID: | 39312195 |
| MONDO: | MONDO:0009696 |
| Tissue: | Bone marrow |
| Tissue Ontology ID: | UBERON:0002371 |
| Cell Source: | ANBL6 |
| CVCL ID: | CVCL_5425 |
| Cell Type: | multiple myeloma cell line |
| Cell Ontology ID: | CL:0007783 |
| Experiment Type: | dCas9-KRAB CRISPRi, CRISPR-Cas9 binding site knockout, dual-luciferase reporter assay, Micro-C chromatin contact validation |
| High Throughput Method: | H3K27ac ChIP-seq, ATAC-seq, WGS, RNA-seq, pooled CRISPRi tiling screen |
| Low Throughput Method: | qRT-PCR, sgRNA lentiviral transduction, dual-luciferase quantification, clonal cell culture |
About Target Gene
| Target gene : | MYC |
About TF
| TF name : | IRF4 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| IRF4 | Calcineurin-regulated NFAT-dependent transcription in lymphocytes | pid | 50 |
| IRF4 | IL4-mediated signaling events | pid | 66 |
| IRF4 | Interferon alpha/beta signaling | reactome | 67 |
| IRF4 | Interferon gamma signaling | reactome | 88 |
| IRF4 | Hs_B_Cell_Receptor_Signaling_Pathway_WP23_89900 | wikipathways | 71 |
Enhancer Associated Network
Expression of Target Genes for the Enhancer