About Enhancer
| Enhancer ID: | E_00799 |
| Species: | Homo sapiens |
| Position : | chr20:5921142-6004055   |
| Year: | 2023 |
| Title: | Enhancer-driven transcription of MCM8 by E2F4 promotes ATR pathway activation and glioma stem cell characteristics |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | glioblastoma |
| PMID: | 37349788 |
| MONDO: | MONDO:0005142 |
| Tissue: | Brain |
| Tissue Ontology ID: | UBERON:0000955 |
| Cell Source: | Glioma stem cell |
| CVCL ID: | |
| Cell Type: | glioma stem cell |
| Cell Ontology ID: | CL:0000127 |
| Experiment Type: | Enhancer activity assay, TF binding assay, Functional perturbation assay |
| High Throughput Method: | ChIP-seq, RNA-seq |
| Low Throughput Method: | ChIP-RT-qPCR, RT-RT-qPCR, Western blot, Sphere Formation Assay, Limiting Dilution Assay, Cell Growth Assay |
About Target Gene
| Target gene : | MCM8 |
About TF
| TF name : | E2F4 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| E2F4 | Cyclin D associated events in G1 | reactome | 38 |
| E2F4 | E2F transcription factor network | pid | 77 |
| E2F4 | G0 and Early G1 | reactome | 25 |
| E2F4 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| E2F4 | Regulation of retinoblastoma protein | pid | 67 |
| E2F4 | SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription | reactome | 32 |
| E2F4 | TGF_beta_Receptor | netpath | 220 |
| E2F4 | TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest | reactome | 18 |
| E2F4 | Cell cycle | kegg | 124 |
| E2F4 | TGF-beta signaling pathway | kegg | 82 |
| E2F4 | Hs_GPCRs,_Class_B_Secretin-like_WP334_79716 | wikipathways | 6 |
| E2F4 | Hs_Tryptophan_metabolism_WP465_85055 | wikipathways | 45 |
| E2F4 | Hs_MAPK_Cascade_WP422_72129 | wikipathways | 10 |
| E2F4 | Hs_TGF-beta_Signaling_Pathway_WP366_90028 | wikipathways | 67 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_00800 | chr20:5921142-6004055 | Brain | neural stem cell | -- |
Expression of Target Genes for the Enhancer