About Enhancer
| Enhancer ID: | E_00922 |
| Species: | Mus musculus |
| Position : | chr16:19007329-19008187   |
| Year: | 2023 |
| Title: | Temporal analyses reveal a pivotal role for sense and antisense enhancer RNAs in coordinate immunoglobulin lambda locus activation. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 37702072 |
| Tissue: | bone marrow |
| Tissue Ontology ID: | UBERON:0002371 |
| Cell Source: | primary pro-/pre-B cells |
| Cell Type: | pro-B cell |
| Cell Ontology ID: | CL:0000826 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Chromatin interaction assay; Endogenous functional perturbation assay |
| High Throughput Method: | Pro-/pre-B ATAC-seq; GRO-seq; MED1 chromatin-data integration |
| Low Throughput Method: | CRISPR/Cas9 deletion spanning PU.1/IRF4 sites; temporal ChIP-qPCR; 3C-qPCR; eRNA perturbation; RT-qPCR |
About Target Gene
| Target gene : | Iglv1 |
About TF
| TF name : | Irf4 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Irf4 | Calcineurin-regulated NFAT-dependent transcription in lymphocytes | pid | 50 |
| Irf4 | IL4-mediated signaling events | pid | 66 |
| Irf4 | Interferon alpha/beta signaling | reactome | 67 |
| Irf4 | Interferon gamma signaling | reactome | 88 |
| Irf4 | Hs_B_Cell_Receptor_Signaling_Pathway_WP23_89900 | wikipathways | 71 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
7 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_00923 | chr16:19007329-19008187 | bone marrow | pro-B cell | NA |
| E_00955 | chr16:19007329-19008187 | bone marrow | pro-B cell | NA |
| E_00956 | chr16:19007329-19008187 | bone marrow | pro-B cell | NA |
| E_04050 | chr16:19007329-19008187 | bone marrow | precursor B cell | NA |
| E_04051 | chr16:19007329-19008187 | bone marrow | precursor B cell | NA |
| E_04052 | chr16:19007329-19008187 | bone marrow | precursor B cell | NA |
| E_04053 | chr16:19007329-19008187 | bone marrow | precursor B cell | NA |
Expression of Target Genes for the Enhancer