About Enhancer
| Enhancer ID: | E_00923 |
| Species: | Mus musculus |
| Position : | chr16:19007329-19008187   |
| Year: | 2023 |
| Title: | Temporal analyses reveal a pivotal role for sense and antisense enhancer RNAs in coordinate immunoglobulin lambda locus activation. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 37702072 |
| Tissue: | bone marrow |
| Tissue Ontology ID: | UBERON:0002371 |
| Cell Source: | primary pro-/pre-B cells |
| Cell Type: | pro-B cell |
| Cell Ontology ID: | CL:0000826 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Chromatin interaction assay; Endogenous functional perturbation assay |
| High Throughput Method: | Pro-/pre-B ATAC-seq; GRO-seq; MED1 chromatin-data integration |
| Low Throughput Method: | CRISPR/Cas9 deletion spanning PU.1/IRF4 sites; temporal ChIP-qPCR; 3C-qPCR; eRNA perturbation; RT-qPCR |
About Target Gene
| Target gene : | Iglv1 |
About TF
| TF name : | Spi1 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Spi1 | C-MYB transcription factor network | pid | 87 |
| Spi1 | ERK cascade ( FGF8 signaling (Mouse) ) | inoh | 17 |
| Spi1 | ERK cascade ( FGF8 signaling (Xenopus) ) | inoh | 17 |
| Spi1 | FGF8 signaling pathway(Mouse) ( FGF8 signaling (Mouse) ) | inoh | 20 |
| Spi1 | FGF8 signaling pathway(Xenopus) ( FGF8 signaling (Xenopus) ) | inoh | 18 |
| Spi1 | Glucocorticoid receptor regulatory network | pid | 85 |
| Spi1 | IL4-mediated signaling events | pid | 66 |
| Spi1 | IL4 | netpath | 75 |
| Spi1 | Interleukin signaling pathway | panther | 86 |
| Spi1 | RANKL | netpath | 84 |
| Spi1 | Regulation of retinoblastoma protein | pid | 67 |
| Spi1 | Validated targets of C-MYC transcriptional repression | pid | 63 |
| Spi1 | Pathways in cancer | kegg | 321 |
| Spi1 | Acute myeloid leukemia | kegg | 53 |
| Spi1 | Hs_RANKL-RANK_(Receptor_activator_of_NFKB_(ligand))_Signaling_Pathway_WP2018_90016 | wikipathways | 26 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
7 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_00922 | chr16:19007329-19008187 | bone marrow | pro-B cell | NA |
| E_00955 | chr16:19007329-19008187 | bone marrow | pro-B cell | NA |
| E_00956 | chr16:19007329-19008187 | bone marrow | pro-B cell | NA |
| E_04050 | chr16:19007329-19008187 | bone marrow | precursor B cell | NA |
| E_04051 | chr16:19007329-19008187 | bone marrow | precursor B cell | NA |
| E_04052 | chr16:19007329-19008187 | bone marrow | precursor B cell | NA |
| E_04053 | chr16:19007329-19008187 | bone marrow | precursor B cell | NA |
Expression of Target Genes for the Enhancer