About Enhancer
| Enhancer ID: | E_01157 |
| Species: | Mus musculus |
| Position : | chr8:121495079-121495626   |
| Year: | 2019 |
| Title: | Cryptic activation of an Irf8 enhancer governs cDC1 fate specification. |
| Genome Build: | mm39 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 31406378 |
| Tissue: | spleen |
| Tissue Ontology ID: | UBERON:0002106 |
| Cell Source: | primary CD24+ cDC1 |
| Cell Type: | classical dendritic cell type 1 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Chromatin accessibility assay; Functional perturbation assay; In vivo enhancer deletion assay |
| High Throughput Method: | ATAC-seq; BATF3 ChIP-seq; IRF8 ChIP-seq; p300 ChIP-seq; chromatin profiling |
| Low Throughput Method: | Retroviral enhancer reporter assay; AICE site-directed mutagenesis; CRISPR/Cas9 enhancer deletion; RT-qPCR; flow cytometry; Flt3L bone-marrow differentiation assay; CRISPR-Cas9 |
About Target Gene
| Target gene : | Irf8 |
About TF
| TF name : | Irf8 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Irf8 | GMCSF-mediated signaling events | pid | 38 |
| Irf8 | Interferon alpha/beta signaling | reactome | 67 |
| Irf8 | Interferon gamma signaling | reactome | 88 |
| Irf8 | Validated targets of C-MYC transcriptional repression | pid | 63 |
| Irf8 | Hs_Type_II_interferon_signaling_(IFNG)_WP619_71168 | wikipathways | 10 |
Enhancer Associated Network
Expression of Target Genes for the Enhancer