About Enhancer
| Enhancer ID: | E_01164 |
| Species: | Mus musculus |
| Position : | chr8:120777048-120777744   |
| Year: | 2019 |
| Title: | Cryptic activation of an Irf8 enhancer governs cDC1 fate specification. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 31406378 |
| Tissue: | bone marrow |
| Tissue Ontology ID: | UBERON:0002371 |
| Cell Source: | Primary mouse bone-marrow MDP/CDP/pre-cDC1 populations |
| Cell Type: | Common dendritic progenitor |
| Cell Ontology ID: | CL:0001029 |
| Experiment Type: | Chromatin-state mapping; TF-binding analysis; endogenous enhancer deletion; lineage differentiation assay; Endogenous enhancer perturbation |
| High Throughput Method: | ATAC-seq; H3K27ac/H3K4me1 ChIP-seq; E2A/TCF3 ChIP-seq integration; transcriptome profiling |
| Low Throughput Method: | CRISPR-Cas9 enhancer deletion in mice; Flt3L bone-marrow culture; flow cytometry; bone-marrow chimera; enhancer reporter assay; CRISPR-Cas9 endogenous deletion/editing |
About Target Gene
| Target gene : | Irf8 |
About TF
| TF name : | Tcf3 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Tcf3 | CDO in myogenesis | reactome | 29 |
| Tcf3 | ID | netpath | 38 |
| Tcf3 | Notch-mediated HES/HEY network | pid | 48 |
| Tcf3 | Notch | netpath | 76 |
| Tcf3 | p38 signaling mediated by MAPKAP kinases | pid | 21 |
| Tcf3 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Tcf3 | Wnt | netpath | 118 |
| Tcf3 | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
3 found
Expression of Target Genes for the Enhancer