About Enhancer
| Enhancer ID: | E_01263 |
| Species: | Mus musculus |
| Position : | chr3:34755799-34762535   |
| Year: | 2014 |
| Title: | CRISPR reveals a distal super-enhancer required for Sox2 expression in mouse embryonic stem cells. |
| Genome Build: | mm10 |
| Enhancer Type: | Super-enhancer |
| Condition: | Normal |
| PMID: | 25486255 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | F123 mouse embryonic stem cells |
| Cell Type: | embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | Super-enhancer activity assay; TF binding assay; Chromatin interaction assay; Functional perturbation assay; Endogenous enhancer perturbation; In vivo functional validation |
| High Throughput Method: | H3K27ac ChIP-seq; H3K4me3 ChIP-seq; RNA-seq; published POU5F1/SOX2/NANOG/MED1 ChIP-seq and Hi-C/5C data integration |
| Low Throughput Method: | Double-CRISPR/Cas9 enhancer deletion; deletion-junction PCR; Sanger sequencing; genomic qPCR; allele-specific RT-qPCR; qRT-PCR; CRISPR-Cas9 endogenous deletion/editing; Mouse genetic deletion/knock-in |
About Target Gene
| Target gene : | Sox2 |
About TF
| TF name : | Sox2 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Sox2 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sox2 | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| Sox2 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| Sox2 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| Sox2 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| Sox2 | Hs_Dopaminergic_Neurogenesis_WP2855_87239 | wikipathways | 19 |
| Sox2 | Hs_Wnt_Signaling_Pathway_and_Pluripotency_WP399_90291 | wikipathways | 12 |
| Sox2 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
| Sox2 | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
15 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01316 | chr3:34743178-34776096 | NA | NA | NA |
| E_01317 | chr3:34743178-34776096 | NA | NA | NA |
| E_01318 | chr3:34743178-34776096 | NA | NA | NA |
| E_01385 | chr3:34752523-34766449 | embryo | embryonic stem cell | NA |
| E_01386 | chr3:34752523-34766449 | embryo | embryonic stem cell | NA |
| E_01387 | chr3:34752523-34766449 | embryo | embryonic stem cell | NA |
| E_01388 | chr3:34752523-34766449 | embryo | embryonic stem cell | NA |
| E_01439 | chr3:34752523-34766449 | embryo | embryonic stem cell | NA |
| E_01261 | chr3:34755799-34762535 | embryo | embryonic stem cell | NA |
| E_01262 | chr3:34755799-34762535 | embryo | embryonic stem cell | NA |
| E_04407 | chr3:34756748-34761748 | embryo | mouse embryonic stem cell | NA |
| E_04995 | chr3:34757100-34758037 | embryoid body | pluripotent stem cell | NA |
| E_05017 | chr3:34757100-34758037 | NA | embryonic stem cell | NA |
| E_04996 | chr3:34761100-34762000 | embryoid body | pluripotent stem cell | NA |
| E_05018 | chr3:34761100-34762000 | NA | embryonic stem cell | NA |
Expression of Target Genes for the Enhancer