About Enhancer
| Enhancer ID: | E_01266 |
| Species: | Mus musculus |
| Position : | chr3:34652359-34665321   |
| Year: | 2014 |
| Title: | CRISPR reveals a distal super-enhancer required for Sox2 expression in mouse embryonic stem cells. |
| Genome Build: | mm9 |
| Enhancer Type: | Super-enhancer |
| Condition: | Normal |
| PMID: | 25486255 |
| Tissue: | blastocyst |
| Tissue Ontology ID: | UBERON:0000358 |
| Cell Source: | F123 |
| CVCL ID: | CVCL_VC43 |
| Cell Type: | embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | Super-enhancer functional validation; TF binding assay; Chromatin interaction analysis; Functional perturbation assay; Gene expression assay; Epigenomic profiling |
| High Throughput Method: | H3K27ac ChIP-seq; H3K4me3 ChIP-seq; Sox2 ChIP-seq reanalysis; RNA-seq; Hi-C reanalysis |
| Low Throughput Method: | CRISPR/Cas9 double-sgRNA super-enhancer deletion; PCR genotyping; qPCR copy-number genotyping; Sanger sequencing; allele-specific RT-qPCR; Western blot; alkaline phosphatase staining; cell proliferation assay |
About Target Gene
| Target gene : | Sox2 |
About TF
| TF name : | Sox2 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Sox2 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sox2 | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| Sox2 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| Sox2 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| Sox2 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| Sox2 | Hs_Dopaminergic_Neurogenesis_WP2855_87239 | wikipathways | 19 |
| Sox2 | Hs_Wnt_Signaling_Pathway_and_Pluripotency_WP399_90291 | wikipathways | 12 |
| Sox2 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
| Sox2 | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
Enhancer Associated Network
Expression of Target Genes for the Enhancer