About Enhancer
| Enhancer ID: | E_01379 |
| Species: | Mus musculus |
| Position : | chr2:45279107-45280206   |
| Year: | 2022 |
| Title: | Ablation of cDC2 development by triple mutations within the Zeb2 enhancer. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 35732734 |
| Tissue: | bone marrow |
| Tissue Ontology ID: | UBERON:0002371 |
| Cell Source: | Hoxb8-immortalized DC precursor system used for mechanistic assays |
| Cell Type: | Common dendritic progenitor |
| Cell Ontology ID: | CL:0001029 |
| Experiment Type: | TF-binding assay; endogenous enhancer mutagenesis; lineage differentiation assay; Endogenous enhancer perturbation; TF binding assay; Chromatin-state profiling; In vivo functional validation |
| High Throughput Method: | CUT&RUN; ChIP-seq; RNA-seq |
| Low Throughput Method: | EMSA; enhancer reporter assay; CRISPR-Cas9 triple motif editing in mice; flow cytometry; progenitor differentiation; CRISPR-Cas9 endogenous deletion/editing; ChIP; In vivo phenotyping |
About Target Gene
| Target gene : | Zeb2 |
About TF
| TF name : | Cebpb |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Cebpb | C-MYB transcription factor network | pid | 87 |
| Cebpb | EGFR1 | netpath | 475 |
| Cebpb | FOXA1 transcription factor network | pid | 45 |
| Cebpb | FOXA2 and FOXA3 transcription factor networks | pid | 45 |
| Cebpb | IFN-gamma pathway | pid | 43 |
| Cebpb | IL3-mediated signaling events | pid | 27 |
| Cebpb | IL4-mediated signaling events | pid | 66 |
| Cebpb | IL4 | netpath | 75 |
| Cebpb | IL6-mediated signaling events | pid | 48 |
| Cebpb | IL6 | netpath | 85 |
| Cebpb | RANKL | netpath | 84 |
| Cebpb | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Cebpb | Regulation of retinoblastoma protein | pid | 67 |
| Cebpb | Senescence-Associated Secretory Phenotype (SASP) | reactome | 108 |
| Cebpb | Signaling mediated by p38-alpha and p38-beta | pid | 35 |
| Cebpb | TCR | netpath | 261 |
| Cebpb | Transcriptional regulation of white adipocyte differentiation | reactome | 79 |
| Cebpb | Validated nuclear estrogen receptor alpha network | pid | 65 |
| Cebpb | Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193 | wikipathways | 62 |
| Cebpb | Hs_Oncostatin_M_Signaling_Pathway_WP2374_73668 | wikipathways | 44 |
| Cebpb | Hs_Transcriptional_activation_by_NRF2_WP3_79527 | wikipathways | 6 |
| Cebpb | Hs_Lung_fibrosis_WP3624_91475 | wikipathways | 23 |
| Cebpb | Hs_IL17_signaling_pathway_WP2112_89838 | wikipathways | 20 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
11 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01348 | chr2:45279107-45280206 | bone marrow | Common dendritic progenitor | NA |
| E_01349 | chr2:45279107-45280206 | bone marrow | Common dendritic progenitor | NA |
| E_01350 | chr2:45279107-45280206 | bone marrow | Common dendritic progenitor | NA |
| E_01378 | chr2:45279107-45280206 | bone marrow | Common dendritic progenitor | NA |
| E_01380 | chr2:45279107-45280206 | bone marrow | Common dendritic progenitor | NA |
| E_01400 | chr2:45279107-45280206 | bone marrow | common dendritic progenitor | NA |
| E_01401 | chr2:45279107-45280206 | bone marrow | common dendritic progenitor | NA |
| E_01402 | chr2:45279107-45280206 | bone marrow | common dendritic progenitor | NA |
| E_01411 | chr2:45279107-45280206 | bone marrow | common dendritic progenitor | NA |
| E_01412 | chr2:45279107-45280206 | bone marrow | common dendritic progenitor | NA |
| E_01413 | chr2:45279107-45280206 | bone marrow | common dendritic progenitor | NA |
Expression of Target Genes for the Enhancer