Comprehensive information about experimentally validated enhancers
| Enhancer ID: | E_01565 |
| Species: | Mus musculus |
| Position : | chr12:86497006-86503356 |
| Year: | 2021 |
| Title: | Enhancer-associated H3K4 methylation safeguards in vitro germline competence. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 34599190 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | ES-R1 |
| CVCL ID: | CVCL_2167 |
| Cell Type: | epiblast-like cell |
| Experiment Type: | Enhancer chromatin-state profiling; functional enhancer deletion; germline-competence assay |
| High Throughput Method: | H3K4me1/H3K4me2/H3K4me3/H3K27ac ChIP-seq; ATAC-seq; RNA-seq; DNA methylation profiling; 4C-seq; Capture Hi-C integration; single-cell RNA-seq |
| Low Throughput Method: | CRISPR-Cas9 enhancer deletion; clonal genotyping/Sanger validation; RT-qPCR; CD15/CD61 flow cytometry |
| Target gene : | Esrrb |
| TF name : | NA |
| Regulatory State : | Active |
| GeneName | Pathway Name | Source | Gene Number |
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