About Enhancer
| Enhancer ID: | E_01599 |
| Species: | Mus musculus |
| Position : | chr10:118242449-118243449   |
| Year: | 2025 |
| Title: | Essential roles of CTCF binding sites at TAD boundaries in modulating chromatin interactions and transcriptional regulation at the Ifng locus. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 41080543 |
| Tissue: | spleen |
| Tissue Ontology ID: | UBERON:0002106 |
| Cell Source: | CD4+ Th1 cells |
| Cell Type: | CD4+ T helper 1 cell |
| Experiment Type: | Enhancer activity assay; chromatin interaction assay; functional perturbation assay |
| High Throughput Method: | CTCF ChIP-seq; RNA-seq; Hi-C; Region Capture Micro-C; 3C |
| Low Throughput Method: | Luciferase reporter assay; 3C-qPCR; ChIP-qPCR; CRISPR-Cas9 perturbation; CRISPR-Cas9 |
About Target Gene
| Target gene : | Ifng |
About TF
| TF name : | Tbx21 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Tbx21 | Calcineurin-regulated NFAT-dependent transcription in lymphocytes | pid | 50 |
| Tbx21 | Glucocorticoid receptor regulatory network | pid | 85 |
| Tbx21 | IL12-mediated signaling events | pid | 62 |
| Tbx21 | IL12 signaling mediated by STAT4 | pid | 33 |
| Tbx21 | IL27-mediated signaling events | pid | 26 |
| Tbx21 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
No overlapping enhancers found in mm10 at this locus.
Expression of Target Genes for the Enhancer