About Enhancer
| Enhancer ID: | E_01604 |
| Species: | Mus musculus |
| Position : | chr11:112482190-112483273   |
| Year: | 2024 |
| Title: | Chromatin profiling identifies chondrocyte-specific Sox9 enhancers important for skeletal development. |
| Genome Build: | mm9 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 38855864 |
| Tissue: | cartilage tissue |
| Tissue Ontology ID: | UBERON:0002418 |
| Cell Source: | Primary rib chondrocytes from newborn mice |
| Cell Type: | chondrocyte |
| Cell Ontology ID: | CL:0000138 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Functional perturbation assay; Endogenous enhancer perturbation; In vivo functional validation |
| High Throughput Method: | ATAC-seq; H3K27ac ChIP-seq; H3K4me2 ChIP-seq; RNA-seq |
| Low Throughput Method: | Luciferase Reporter Assay; Pitx1 ChIP-qPCR; RT-qPCR; Western blot; Biotinylated DNA Pull-Down Assay; CRISPR/Cas9 enhancer deletion; genomic PCR; RNA in situ hybridization; Alcian blue/alizarin red S skeletal staining; CRISPR-Cas9 endogenous deletion/editing; Mouse genetic deletion/knock-in |
About Target Gene
| Target gene : | Sox9 |
About TF
| TF name : | Pitx1 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer Associated Network
Expression of Target Genes for the Enhancer