About Enhancer
| Enhancer ID: | E_01781 |
| Species: | Mus musculus |
| Position : | chr7:79042226-79044226   |
| Year: | 2019 |
| Title: | Differential transactivation of the upstream aggrecan enhancer regulated by PAX1/9 depends on SOX9-driven transactivation. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 30872687 |
| Tissue: | intervertebral disk |
| Tissue Ontology ID: | UBERON:0001066 |
| Cell Source: | primary mouse annulus fibrosus cells / chondrocytes |
| Cell Type: | chondrocyte |
| Cell Ontology ID: | CL:0000138 |
| Experiment Type: | Enhancer activity assay; TF-site functional dissection; endogenous enhancer deletion; In vivo functional validation |
| High Throughput Method: | ChIP-seq data integration |
| Low Throughput Method: | Luciferase reporter assay; TF overexpression; motif mutagenesis; ChIP/EMSA; CRISPR-Cas9 deletion of a 695-bp region containing UE; qRT-PCR; In vivo phenotyping; Reporter assay |
About Target Gene
| Target gene : | Acan |
About TF
| TF name : | Sox9 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Sox9 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sox9 | TGF_beta_Receptor | netpath | 220 |
| Sox9 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
| Sox9 | Hs_Spinal_Cord_Injury_WP2431_87678 | wikipathways | 21 |
Enhancer Associated Network
Expression of Target Genes for the Enhancer