About Enhancer
| Enhancer ID: | E_01885 |
| Species: | Homo sapiens |
| Position : | chr12:7970611-7971032   |
| Year: | 2023 |
| Title: | Dynamic network-guided CRISPRi screen identifies CTCF-loop-constrained nonlinear enhancer gene regulatory activity during cell state transitions |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| Disease Name: | -- |
| PMID: | 37488417 |
| MONDO: | |
| Tissue: | Inner cell mass |
| Tissue Ontology ID: | UBERON:0000087 |
| Cell Source: | HUES8 human embryonic stem cell line |
| CVCL ID: | CVCL_2063 |
| Cell Type: | embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | CRISPR interference-based functional screen |
| High Throughput Method: | CRISPRi screen, RNA-seq, ATAC-seq, ChIP-seq, Hi-C |
| Low Throughput Method: | RT-qPCR, flow cytometry, FACS |
About Target Gene
| Target gene : | NANOG |
About TF
| TF name : | OCT4SOX2NANOG |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| SOX2 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| SOX2 | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| SOX2 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| SOX2 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| SOX2 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| SOX2 | Hs_Dopaminergic_Neurogenesis_WP2855_87239 | wikipathways | 19 |
| SOX2 | Hs_Wnt_Signaling_Pathway_and_Pluripotency_WP399_90291 | wikipathways | 12 |
| SOX2 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
| SOX2 | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
| NANOG | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| NANOG | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| NANOG | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
No overlapping enhancers found in hg38 at this locus.
Expression of Target Genes for the Enhancer