About Enhancer
| Enhancer ID: | E_02038 |
| Species: | Homo sapiens |
| Position : | chr5:131406243-131406930   |
| Year: | 2004 |
| Title: | Granulocyte-macrophage colony-stimulating factor enhancer activation requires cooperation between NFAT and AP-1 elements and is associated with extensive nucleosome reorganization. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 15340054 |
| Tissue: | blood |
| Tissue Ontology ID: | UBERON:0000178 |
| Cell Source: | Jurkat |
| CVCL ID: | CVCL_0065 |
| Cell Type: | T cell |
| Cell Ontology ID: | CL:0000084 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Functional perturbation assay; Chromatin accessibility assay; Chromatin remodeling assay; In vivo functional validation |
| Low Throughput Method: | Site-directed mutagenesis; transient transfection; luciferase reporter assay; PMA/A23187 stimulation; EMSA; recombinant protein EMSA; DMS in vivo footprinting; LM-PCR; DNase I hypersensitivity assay; DNase I footprinting; MNase nucleosome mapping; restriction enzyme accessibility assay; stable transfection; In vivo phenotyping |
About Target Gene
| Target gene : | CSF2 |
About TF
| TF name : | NFATC2 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| NFATC2 | AP-1 transcription factor network | pid | 71 |
| NFATC2 | Axon guidance mediated by netrin | panther | 30 |
| NFATC2 | BCR | netpath | 161 |
| NFATC2 | Calcineurin-regulated NFAT-dependent transcription in lymphocytes | pid | 50 |
| NFATC2 | Calcium signaling in the CD4+ TCR pathway | pid | 29 |
| NFATC2 | CLEC7A (Dectin-1) induces NFAT activation | reactome | 13 |
| NFATC2 | Downstream signaling in naïve CD8+ T cells | pid | 69 |
| NFATC2 | Fc-epsilon receptor I signaling in mast cells | pid | 64 |
| NFATC2 | FCERI mediated Ca+2 mobilization | reactome | 41 |
| NFATC2 | Glucocorticoid receptor regulatory network | pid | 85 |
| NFATC2 | IL12 signaling mediated by STAT4 | pid | 33 |
| NFATC2 | IL2 signaling events mediated by PI3K | pid | 38 |
| NFATC2 | Inflammation mediated by chemokine and cytokine signaling pathway | panther | 189 |
| NFATC2 | KitReceptor | netpath | 104 |
| NFATC2 | Noncanonical Wnt signaling pathway | pid | 33 |
| NFATC2 | Role of Calcineurin-dependent NFAT signaling in lymphocytes | pid | 54 |
| NFATC2 | Signaling by Wnt | reactome | 10 |
| NFATC2 | TCR | netpath | 261 |
| NFATC2 | Validated transcriptional targets of AP1 family members Fra1 and Fra2 | pid | 37 |
| NFATC2 | Wnt | netpath | 118 |
| NFATC2 | Wnt signaling pathway | panther | 250 |
| NFATC2 | Wnt signaling pathway | kegg | 135 |
| NFATC2 | Axon guidance | kegg | 126 |
| NFATC2 | VEGF signaling pathway | kegg | 70 |
| NFATC2 | Natural killer cell mediated cytotoxicity | kegg | 125 |
| NFATC2 | T cell receptor signaling pathway | kegg | 105 |
| NFATC2 | B cell receptor signaling pathway | kegg | 70 |
| NFATC2 | Hs_T-Cell_antigen_Receptor_(TCR)_pathway_during_Staphylococcus_aureus_infection_WP3863_90702 | wikipathways | 35 |
| NFATC2 | Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000 | wikipathways | 153 |
| NFATC2 | Hs_Cardiac_Hypertrophic_Response_WP2795_85087 | wikipathways | 23 |
| NFATC2 | Hs_T-Cell_antigen_Receptor_(TCR)_Signaling_Pathway_WP69_89937 | wikipathways | 60 |
| NFATC2 | Hs_B_Cell_Receptor_Signaling_Pathway_WP23_89900 | wikipathways | 71 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
8 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02035 | chr5:131406243-131406930 | blood | T cell | NA |
| E_02036 | chr5:131406243-131406930 | blood | T cell | NA |
| E_02037 | chr5:131406243-131406930 | blood | T cell | NA |
| E_03687 | chr5:131406243-131406930 | blood | T cell | NA |
| E_03688 | chr5:131406243-131406930 | blood | T cell | NA |
| E_04044 | chr5:131406243-131406930 | blood | T cell | NA |
| E_04598 | chr5:131406243-131406930 | blood | T cell | NA |
| E_04599 | chr5:131406243-131406930 | blood | T cell | NA |
Expression of Target Genes for the Enhancer