About Enhancer
| Enhancer ID: | E_02130 |
| Species: | Mus musculus |
| Position : | chr4:135009700-135010200   |
| Year: | 2016 |
| Title: | NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| Disease Name: | -- |
| PMID: | 26751055 |
| MONDO: | |
| Tissue: | Embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | Mouse embryonic stem cells |
| CVCL ID: | |
| Cell Type: | mouse embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | Enhancer activity assay, TF binding assay, Functional perturbation assay, Epigenetic modification assay |
| High Throughput Method: | ChIP-seq, Microarray |
| Low Throughput Method: | Luciferase reporter assay, ChIP-RT-qPCR, RT-RT-qPCR, CRISPR-Cas9 knockout, Western blot, Immunofluorescence, Flow cytometry, Blastocyst injection assay |
About Target Gene
| Target gene : | Prdm14 |
About TF
| TF name : | Nanog |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Nanog | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| Nanog | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| Nanog | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
4 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02131 | chr4:135009700-135010200 | Embryo | epiblast-like cell | -- |
| E_02132 | chr4:135009700-135010200 | Embryo | primordial germ cell-like cell | -- |
| E_02333 | chr4:135010000-135010100 | Embryo | epiblast-like cell | -- |
| E_02334 | chr4:135010000-135010100 | Embryo | primordial germ cell-like cell | -- |
Expression of Target Genes for the Enhancer