About Enhancer
| Enhancer ID: | E_02180 |
| Species: | Rattus norvegicus |
| Position : | chr7:84833410-84834080   |
| Year: | 2017 |
| Title: | A Novel Regulatory Mechanism of Type II Collagen Expression via a SOX9-dependent Enhancer in Intron 6. |
| Genome Build: | rn4 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 27881681 |
| Tissue: | cartilage tissue |
| Tissue Ontology ID: | UBERON:0002418 |
| Cell Source: | RCS rat chondrosarcoma cells |
| Cell Type: | chondrocyte |
| Cell Ontology ID: | CL:0000138 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Endogenous functional perturbation assay; Endogenous enhancer perturbation; Chromatin-state profiling |
| High Throughput Method: | SOX9 ChIP-seq; ChIP-seq |
| Low Throughput Method: | Luciferase reporter assay; stable reporter assay; CRISPR/Cas9 intron-6 deletion; ChIP-qPCR; EMSA/supershift; RT-qPCR; Western blot; CRISPR-Cas9 endogenous deletion/editing; EMSA; ChIP |
About Target Gene
| Target gene : | Col2a1 |
About TF
| TF name : | Sox9 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Sox9 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sox9 | TGF_beta_Receptor | netpath | 220 |
| Sox9 | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
| Sox9 | Hs_Spinal_Cord_Injury_WP2431_87678 | wikipathways | 21 |
Enhancer Associated Network
Overlapping Enhancers (rn4)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02179 | chr7:84833410-84834080 | cartilage tissue | chondrocyte | NA |
Expression of Target Genes for the Enhancer