About Enhancer
| Enhancer ID: | E_02190 |
| Species: | Homo sapiens |
| Position : | chr5:173245543-173245843   |
| Year: | 2023 |
| Title: | An Anterior Second Heart Field Enhancer Regulates the Gene Regulatory Network of the Cardiac Outflow Tract. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 37772400 |
| Tissue: | heart |
| Tissue Ontology ID: | UBERON:0000948 |
| Cell Source: | hiPSC-derived cardiomyocytes |
| Cell Type: | cardiac muscle cell |
| Cell Ontology ID: | CL:0000746 |
| Experiment Type: | TF binding assay; chromatin accessibility assay; cross-species enhancer validation; Endogenous enhancer perturbation; Chromatin-state profiling; In vivo functional validation |
| High Throughput Method: | GATA6 ChIP-seq; ATAC-seq; single-cell RNA-seq; RNA-seq; ChIP-seq |
| Low Throughput Method: | ChIP-qPCR / targeted validation; comparison with mouse CRISPR-deletion phenotype; CRISPR-Cas9 endogenous deletion/editing; ChIP; Site-directed/motif mutagenesis; Mouse genetic deletion/knock-in |
About Target Gene
| Target gene : | NKX2-5 |
About TF
| TF name : | GATA6 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| GATA6 | Factors involved in megakaryocyte development and platelet production | reactome | 111 |
| GATA6 | Notch-mediated HES/HEY network | pid | 48 |
| GATA6 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| GATA6 | Surfactant metabolism | reactome | 30 |
| GATA6 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
Enhancer Associated Network
Overlapping Enhancers (hg38)
No overlapping enhancers found in hg38 at this locus.
Expression of Target Genes for the Enhancer