About Enhancer
| Enhancer ID: | E_02217 |
| Species: | Homo sapiens |
| Position : | chr12:8122645-8123945   |
| Year: | 2012 |
| Title: | Dynamic change of chromatin conformation in response to hypoxia enhances the expression of GLUT3 (SLC2A3) by cooperative interaction of hypoxia-inducible factor 1 and KDM3A. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 22645302 |
| Tissue: | umbilical vein |
| Tissue Ontology ID: | UBERON:0002066 |
| Cell Source: | HEK293 for HIF1 overexpression reporter assays |
| CVCL ID: | CVCL_0045 |
| Cell Type: | endothelial cell of umbilical vein |
| Cell Ontology ID: | CL:0002618 |
| Experiment Type: | SLC2A3 distal enhancer 1 (~35 kb upstream); strong HIF1 reporter/3C evidence in 2012; later work shows overlap with the HIF-responsive NICI promoter and supports an additional lncRNA-mediated mechanism |
| High Throughput Method: | HIF1 ChIP-seq; genome-wide H3K27ac profiling/ChIP-seq; DNA microarray/time-course transcriptome profiling |
| Low Throughput Method: | ChIP-qPCR; luciferase reporter; HIF1A siRNA/overexpression; KDM3A knockdown; HRE site-directed mutagenesis; 3C/3C-ChIP; RT-qPCR |
About Target Gene
| Target gene : | SLC2A3 |
About TF
| TF name : | HIF1A |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| HIF1A | Angiogenesis | panther | 141 |
| HIF1A | AP-1 transcription factor network | pid | 71 |
| HIF1A | BMAL1:CLOCK,NPAS2 activates circadian gene expression | reactome | 42 |
| HIF1A | Circadian Clock | reactome | 49 |
| HIF1A | HIF-1-alpha transcription factor network | pid | 67 |
| HIF1A | Hypoxia response via HIF activation | panther | 16 |
| HIF1A | Hypoxic and oxygen homeostasis regulation of HIF-1-alpha | pid | 19 |
| HIF1A | Notch-mediated HES/HEY network | pid | 48 |
| HIF1A | NOTCH1 Intracellular Domain Regulates Transcription | reactome | 47 |
| HIF1A | Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha | reactome | 18 |
| HIF1A | Parkinson disease | panther | 85 |
| HIF1A | PTK6 Expression | reactome | 5 |
| HIF1A | PTK6 promotes HIF1A stabilization | reactome | 6 |
| HIF1A | Regulation of gene expression by Hypoxia-inducible Factor | reactome | 10 |
| HIF1A | VEGF signaling pathway | panther | 54 |
| HIF1A | VEGFR1 specific signals | pid | 29 |
| HIF1A | mTOR signaling pathway | kegg | 50 |
| HIF1A | Pathways in cancer | kegg | 321 |
| HIF1A | Renal cell carcinoma | kegg | 65 |
| HIF1A | Hs_Hepatitis_C_and_Hepatocellular_Carcinoma_WP3646_88640 | wikipathways | 36 |
| HIF1A | Hs_Focal_Adhesion-PI3K-Akt-mTOR-signaling_pathway_WP3932_91101 | wikipathways | 8 |
| HIF1A | Hs_AGE-RAGE_pathway_WP2324_89798 | wikipathways | 27 |
| HIF1A | Hs_Notch_Signaling_Pathway_WP61_78592 | wikipathways | 16 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
3 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02203 | chr12:8122645-8123945 | umbilical vein | endothelial cell of umbilical vein | NA |
| E_02209 | chr12:8122645-8123945 | vascular endothelium / kidney-derived cell-line context | endothelial cell / epithelial-like kidney-derived cell model | NA |
| E_02223 | chr12:8122645-8123945 | vascular endothelium / kidney-derived cell-line context | endothelial cell / epithelial-like kidney-derived cell model | NA |
Expression of Target Genes for the Enhancer