About Enhancer
| Enhancer ID: | E_02492 |
| Species: | Homo sapiens |
| Position : | chr7:94002773-94005073   |
| Year: | 2015 |
| Title: | Failed degradation of JunB contributes to overproduction of type I collagen and development of dermal fibrosis in patients with systemic sclerosis. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | systemic sclerosis |
| PMID: | 25303440 |
| MONDO: | MONDO:0005100 |
| Tissue: | skin of body |
| Tissue Ontology ID: | UBERON:0002097 |
| Cell Source: | Primary human dermal fibroblasts from systemic sclerosis patients |
| Cell Type: | fibroblast |
| Cell Ontology ID: | CL:0000057 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Functional perturbation assay |
| Low Throughput Method: | Reporter Assay; EMSA/supershift; ChIP-qPCR; AP-1 site mutagenesis; JUNB siRNA knockdown; RT-qPCR; Western blot; EMSA; ChIP |
About Target Gene
| Target gene : | COL1A2 |
About TF
| TF name : | JUNB |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| JUNB | AP-1 transcription factor network | pid | 71 |
| JUNB | ATF-2 transcription factor network | pid | 59 |
| JUNB | BCR | netpath | 161 |
| JUNB | Calcineurin-regulated NFAT-dependent transcription in lymphocytes | pid | 50 |
| JUNB | Calcium signaling in the CD4+ TCR pathway | pid | 29 |
| JUNB | Downstream signaling in naïve CD8+ T cells | pid | 69 |
| JUNB | IL6-mediated signaling events | pid | 48 |
| JUNB | Inflammation mediated by chemokine and cytokine signaling pathway | panther | 189 |
| JUNB | SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription | reactome | 32 |
| JUNB | TGF-beta signaling pathway | panther | 88 |
| JUNB | TGF_beta_Receptor | netpath | 220 |
| JUNB | TNFalpha | netpath | 274 |
| JUNB | Validated transcriptional targets of AP1 family members Fra1 and Fra2 | pid | 37 |
| JUNB | Hs_Kit_receptor_signaling_pathway_WP304_78799 | wikipathways | 46 |
| JUNB | Hs_TGF-beta_Signaling_Pathway_WP366_90028 | wikipathways | 67 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
6 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02526 | chr7:94002773-94005073 | NA | fibroblast | systemic sclerosis |
| E_02527 | chr7:94002773-94005073 | NA | fibroblast | systemic sclerosis |
| E_02600 | chr7:94002773-94005073 | skin of body | fibroblast | systemic sclerosis |
| E_02601 | chr7:94002773-94005073 | connective tissue | fibroblast | systemic sclerosis |
| E_02602 | chr7:94002773-94005073 | connective tissue | fibroblast | systemic sclerosis |
| E_02615 | chr7:94002773-94005073 | lung | fibroblast | NA |
Expression of Target Genes for the Enhancer