About Enhancer
| Enhancer ID: | E_02625 |
| Species: | Homo sapiens |
| Position : | chr13:28840000-28850000   |
| Year: | 2023 |
| Title: | Systematic characterization of the HOXA9 downstream targets in MLL-r leukemia by noncoding CRISPR screens. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | KMT2A-rearranged acute lymphoblastic leukemia |
| PMID: | 38016946 |
| MONDO: | MONDO:0035941 |
| Tissue: | blood |
| Tissue Ontology ID: | UBERON:0000178 |
| Cell Source: | SEM |
| CVCL ID: | CVCL_0095 |
| Cell Type: | precursor B cell |
| Cell Ontology ID: | CL:0000817 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Functional perturbation assay; Chromatin interaction assay |
| High Throughput Method: | noncoding CRISPR screen; HOXA9 ChIP-seq; MEIS1 ChIP-seq; ATAC-seq; RNA-seq; Hi-C; Capture-C; ChIP-seq |
| Low Throughput Method: | Cas9 enhancer editing; CRISPRi; RT-qPCR; Western blot; competitive proliferation assay; FLT3 cDNA rescue; flow cytometry; NSG transplantation |
About Target Gene
| Target gene : | FLT3 |
About TF
| TF name : | MEIS1 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| MEIS1 | Activation of anterior HOX genes in hindbrain development during early embryogenesis | reactome | 120 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
6 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02624 | chr13:28840000-28850000 | blood | precursor B cell | KMT2A-rearranged acute lymphoblastic leukemia |
| E_03831 | chr13:28843000-28843500 | blood | B cell | KMT2A::AFF1-rearranged B-cell acute lymphoblastic leukemia |
| E_03832 | chr13:28843000-28843500 | bone marrow | B-lymphoblast | B-cell acute lymphoblastic leukemia |
| E_03867 | chr13:28843000-28843500 | blood | B cell | KMT2A::AFF1-rearranged B-cell acute lymphoblastic leukemia |
| E_03868 | chr13:28843000-28843500 | blood | B cell | KMT2A::AFF1-rearranged B-cell acute lymphoblastic leukemia |
| E_03869 | chr13:28843000-28843500 | bone marrow | B-lymphoblast | B-cell acute lymphoblastic leukemia |
Expression of Target Genes for the Enhancer