Enhancer Detail

Comprehensive information about experimentally validated enhancers

About Enhancer
Enhancer ID: E_02645
Species: Homo sapiens
Position : chr6:31247578-31247900
Year: 2016
Title: A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening.
Genome Build: hg18
Enhancer Type: Enhancer
Condition: Normal
PMID:  26813977
Tissue: embryo
Tissue Ontology ID: UBERON:0000922
Cell Source: WA01
CVCL ID: CVCL_9771
Cell Type: embryonic stem cell
Cell Ontology ID: CL:0002322
Experiment Type: CRISPR screen; enhancer activity assay; functional perturbation assay; Endogenous enhancer perturbation
High Throughput Method: Pooled CRISPR/Cas9 sgRNA screen; amplicon deep sequencing; 4C-seq; Hi-C data integration; CRISPR genetic screen
Low Throughput Method: Locus-specific CRISPR/Cas9 mutagenesis; luciferase reporter assay; flow cytometry; RT-qPCR; CRISPR-Cas9 endogenous deletion/editing
About Target Gene
Target gene : POU5F1
About TF
TF name : NA
About Regulatory State
Regulatory State : Active
Upstream Pathway Annotation of TF
GeneName Pathway Name Source Gene Number
Enhancer Associated Network
Overlapping Enhancers (hg18)
No overlapping enhancers found in hg18 at this locus.
Expression of Target Genes for the Enhancer