About Enhancer
| Enhancer ID: | E_02654 |
| Species: | Homo sapiens |
| Position : | chr6:31139599-31139921   |
| Year: | 2016 |
| Title: | A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 26813977 |
| Tissue: | embryonic stem-cell culture |
| Cell Source: | WA01 |
| CVCL ID: | CVCL_9771 |
| Cell Type: | embryonic stem cell |
| Cell Ontology ID: | CL:0002322 |
| Experiment Type: | High-throughput cis-regulatory screen; endogenous functional perturbation; enhancer activity assay |
| High Throughput Method: | Pooled CRISPR-Cas9 screen; DNase-seq/ENCODE chromatin-data integration; DNA methylation profiling |
| Low Throughput Method: | Individual CRISPR-Cas9 perturbation/deletion; luciferase reporter assay; flow cytometry; qRT-PCR; CRISPR-Cas9 |
About Target Gene
| Target gene : | POU5F1 |
About TF
| TF name : | POU5F1 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| POU5F1 | HIF-2-alpha transcription factor network | pid | 34 |
| POU5F1 | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| POU5F1 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| POU5F1 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| POU5F1 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| POU5F1 | Hs_Wnt_Signaling_Pathway_and_Pluripotency_WP399_90291 | wikipathways | 12 |
Enhancer Associated Network
Expression of Target Genes for the Enhancer