Enhancer Detail

Comprehensive information about experimentally validated enhancers

About Enhancer
Enhancer ID: E_02662
Species: Homo sapiens
Position : chr6:31126537-31126685
Year: 2016
Title: A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening.
Genome Build: hg19
Enhancer Type: Enhancer
Condition: Normal
PMID:  26813977
Tissue: embryonic stem-cell culture
Cell Source: WA01
CVCL ID: CVCL_9771
Cell Type: embryonic stem cell
Cell Ontology ID: CL:0002322
Experiment Type: High-throughput cis-regulatory screen; endogenous functional perturbation; enhancer activity assay
High Throughput Method: Pooled CRISPR-Cas9 screen; DNase-seq/ENCODE chromatin-data integration; DNA methylation profiling
Low Throughput Method: Individual CRISPR-Cas9 perturbation/deletion; luciferase reporter assay; flow cytometry; qRT-PCR; CRISPR-Cas9
About Target Gene
Target gene : POU5F1
About TF
TF name : NA
About Regulatory State
Regulatory State : Active
Upstream Pathway Annotation of TF
GeneName Pathway Name Source Gene Number
Enhancer Associated Network
Overlapping Enhancers (hg19) 2 found
Enhancer ID Position Tissue Cell Disease
E_02657 chr6:31126537-31126685 embryo embryonic stem cell NA
E_02666 chr6:31126537-31126685 NA NA NA
Expression of Target Genes for the Enhancer