About Enhancer
| Enhancer ID: | E_02682 |
| Species: | Homo sapiens |
| Position : | chr22:24236570-24237410   |
| Year: | 2011 |
| Title: | Identification of a novel cell type-specific intronic enhancer of macrophage migration inhibitory factor (MIF) and its regulation by mithramycin. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | rheumatoid arthritis |
| PMID: | 21087445 |
| MONDO: | MONDO:0008383 |
| Tissue: | layer of synovial tissue |
| Tissue Ontology ID: | UBERON:0007616 |
| Cell Source: | primary rheumatoid-arthritis fibroblast-like synoviocytes / SW982 |
| Cell Type: | fibroblast |
| Cell Ontology ID: | CL:0000057 |
| Experiment Type: | Cell-type-specific intronic enhancer mapping; cis-element mutagenesis; TF-binding assay; TF binding assay |
| Low Throughput Method: | DNase-I hypersensitivity mapping; luciferase reporter assay; GC-box mutagenesis; EMSA; SP1/SP3 RNAi; mithramycin inhibition; siRNA knockdown |
About Target Gene
| Target gene : | MIF |
About TF
| TF name : | SP1 |
About Regulatory State
| Regulatory State : | Inactive |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| SP1 | Activation of gene expression by SREBF (SREBP) | reactome | 42 |
| SP1 | AndrogenReceptor | netpath | 167 |
| SP1 | AP-1 transcription factor network | pid | 71 |
| SP1 | C-MYB transcription factor network | pid | 87 |
| SP1 | Direct p53 effectors | pid | 141 |
| SP1 | E2F transcription factor network | pid | 77 |
| SP1 | EGFR1 | netpath | 475 |
| SP1 | FOXA1 transcription factor network | pid | 45 |
| SP1 | FOXA2 and FOXA3 transcription factor networks | pid | 45 |
| SP1 | FOXM1 transcription factor network | pid | 43 |
| SP1 | HIF-1-alpha transcription factor network | pid | 67 |
| SP1 | HIF-2-alpha transcription factor network | pid | 34 |
| SP1 | Huntington disease | panther | 113 |
| SP1 | IL2 signaling events mediated by STAT5 | pid | 30 |
| SP1 | IL4-mediated signaling events | pid | 66 |
| SP1 | Leptin | netpath | 98 |
| SP1 | Oncogene Induced Senescence | reactome | 30 |
| SP1 | p73 transcription factor network | pid | 80 |
| SP1 | PPARA activates gene expression | reactome | 113 |
| SP1 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| SP1 | Regulation of Telomerase | pid | 70 |
| SP1 | RNA polymerase II transcribes snRNA genes | reactome | 70 |
| SP1 | SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription | reactome | 32 |
| SP1 | TGF_beta_Receptor | netpath | 220 |
| SP1 | Validated targets of C-MYC transcriptional repression | pid | 63 |
| SP1 | Validated transcriptional targets of AP1 family members Fra1 and Fra2 | pid | 37 |
| SP1 | Validated transcriptional targets of TAp63 isoforms | pid | 54 |
| SP1 | TGF-beta signaling pathway | kegg | 82 |
| SP1 | Huntington's disease | kegg | 182 |
| SP1 | Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017 | wikipathways | 41 |
| SP1 | Hs_Leptin_signaling_pathway_WP2034_89856 | wikipathways | 37 |
| SP1 | Hs_Interleukin-11_Signaling_Pathway_WP2332_79525 | wikipathways | 17 |
| SP1 | Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 | wikipathways | 122 |
| SP1 | Hs_Estrogen_signaling_pathway_WP712_78491 | wikipathways | 13 |
| SP1 | Hs_Sudden_Infant_Death_Syndrome_(SIDS)_Susceptibility_Pathways_WP706_86078 | wikipathways | 33 |
| SP1 | Hs_TGF-beta_Signaling_Pathway_WP366_90028 | wikipathways | 67 |
| SP1 | Hs_AGE-RAGE_pathway_WP2324_89798 | wikipathways | 27 |
| SP1 | Hs_Androgen_receptor_signaling_pathway_WP138_79958 | wikipathways | 27 |
| SP1 | Hs_EGF-EGFR_Signaling_Pathway_WP437_79266 | wikipathways | 107 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
15 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_02646 | chr22:24236392-24237411 | blood | T cell | NA |
| E_02677 | chr22:24236392-24237411 | synovium | fibroblast-like synoviocyte | rheumatoid arthritis |
| E_02683 | chr22:24236392-24237411 | blood | monocyte-like cell line | NA |
| E_02691 | chr22:24236392-24237411 | kidney | transformed embryonic kidney-derived cell line | NA |
| E_02693 | chr22:24236392-24237411 | NA | fibroblast-like sarcoma cell line | NA |
| E_02694 | chr22:24236392-24237411 | blood | NA | rheumatoid arthritis |
| E_02695 | chr22:24236392-24237411 | kidney | NA | rheumatoid arthritis |
| E_02696 | chr22:24236392-24237411 | layer of synovial tissue | NA | rheumatoid arthritis |
| E_02697 | chr22:24236392-24237411 | NA | macrophage | rheumatoid arthritis |
| E_02698 | chr22:24236392-24237411 | NA | monocyte | rheumatoid arthritis |
| E_02699 | chr22:24236392-24237411 | NA | macrophage | rheumatoid arthritis |
| E_02700 | chr22:24236392-24237411 | NA | macrophage | rheumatoid arthritis |
| E_02689 | chr22:24236570-24237410 | blood | epithelial cell | NA |
| E_02690 | chr22:24236570-24237410 | blood | monocyte | NA |
| E_02692 | chr22:24236570-24237410 | blood | T lymphoblast | NA |
Expression of Target Genes for the Enhancer