About Enhancer
| Enhancer ID: | E_03055 |
| Species: | Homo sapiens |
| Position : | chrX:49273000-49274000   |
| Year: | 2025 |
| Title: | Genome-wide CRISPR screen in human T cells reveals regulators of FOXP3. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 40140585 |
| Tissue: | blood |
| Tissue Ontology ID: | UBERON:0000178 |
| Cell Source: | Primary human CD4+ T cells differentiated into iTreg cells |
| Cell Type: | regulatory T cell |
| Cell Ontology ID: | CL:0000815 |
| Experiment Type: | TF binding assay; Chromatin accessibility assay; Histone modification assay; Functional perturbation assay |
| High Throughput Method: | Genome-wide CRISPR-Cas9 loss-of-function screen; Perturb-icCITE-seq; icRNA-seq; inChIP-seq; inATAC-seq; CUT&RUN |
| Low Throughput Method: | RBPJ CRISPR-Cas9 knockout; RBPJ overexpression; FOXP3 flow cytometry; Repeated TCR/cytokine restimulation stability assay; T-cell suppression assay |
About Target Gene
| Target gene : | FOXP3 |
About TF
| TF name : | RBPJ |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| RBPJ | Alzheimer disease-presenilin pathway | panther | 98 |
| RBPJ | Angiogenesis | panther | 141 |
| RBPJ | Canonical Notch signaling pathway ( Notch signaling pathway Diagram ) | inoh | 19 |
| RBPJ | Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants | reactome | 57 |
| RBPJ | Constitutive Signaling by NOTCH1 PEST Domain Mutants | reactome | 57 |
| RBPJ | Mammalian Notch signaling pathway ( Notch signaling pathway Diagram ) | inoh | 15 |
| RBPJ | NICD traffics to nucleus | reactome | 13 |
| RBPJ | Notch-HLH transcription pathway | reactome | 13 |
| RBPJ | Notch-mediated HES/HEY network | pid | 48 |
| RBPJ | Notch | netpath | 76 |
| RBPJ | Notch signaling pathway | panther | 27 |
| RBPJ | Notch signaling pathway | pid | 58 |
| RBPJ | NOTCH1 Intracellular Domain Regulates Transcription | reactome | 47 |
| RBPJ | NOTCH2 intracellular domain regulates transcription | reactome | 12 |
| RBPJ | Pre-NOTCH Transcription and Translation | reactome | 29 |
| RBPJ | Presenilin action in Notch and Wnt signaling | pid | 46 |
| RBPJ | Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells | reactome | 7 |
| RBPJ | Notch signaling pathway | kegg | 44 |
| RBPJ | Hs_Neural_Crest_Differentiation_WP2064_79263 | wikipathways | 40 |
Enhancer Associated Network
Expression of Target Genes for the Enhancer