About Enhancer
| Enhancer ID: | E_03065 |
| Species: | Homo sapiens |
| Position : | chrX:49260470-49261090   |
| Year: | 2025 |
| Title: | Genome-wide CRISPR screen in human T cells reveals regulators of FOXP3. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 40140585 |
| Tissue: | blood |
| Tissue Ontology ID: | UBERON:0000178 |
| Cell Source: | Primary human CD4+ T cells differentiated into iTreg cells |
| Cell Type: | regulatory T cell |
| Cell Ontology ID: | CL:0000815 |
| Experiment Type: | Functional perturbation assay; Enhancer epigenetic-state assay; Chromatin accessibility assay |
| High Throughput Method: | Genome-wide CRISPR-Cas9 loss-of-function screen; Perturb-icCITE-seq; icRNA-seq; inChIP-seq; inATAC-seq; H3K9ac/H3K27ac ChIP-seq |
| Low Throughput Method: | RBPJ CRISPR-Cas9 knockout; RBPJ overexpression; Bisulfite sequencing of FOXP3 CNS2 CpGs; FOXP3 flow cytometry; Repeated TCR/cytokine restimulation stability assay; T-cell suppression assay |
About Target Gene
| Target gene : | FOXP3 |
About TF
| TF name : | NA |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer Associated Network
Expression of Target Genes for the Enhancer