About Enhancer
| Enhancer ID: | E_03066 |
| Species: | Homo sapiens |
| Position : | chr20:21576001-21577647   |
| Year: | 2025 |
| Title: | p300/CBP is an essential driver of pathogenic enhancer activity and gene expression in Ewing sarcoma. |
| Genome Build: | hg38 |
| Enhancer Type: | Enhancer |
| Condition: | Disease |
| Disease Name: | Ewing sarcoma |
| PMID: | 40890402 |
| MONDO: | MONDO:0012817 |
| Tissue: | muscle organ |
| Tissue Ontology ID: | UBERON:0001630 |
| Cell Source: | A-673-dHALO and A-673 EWSR1::FLI1-knockout derivatives |
| Cell Type: | Ewing sarcoma cell |
| Experiment Type: | Enhancer-associated functional perturbation assay |
| High Throughput Method: | EWSR1::FLI1 ChIP-seq; EP300 ChIP-seq; CREBBP ChIP-seq; H3K27ac ChIP-seq; ribo-depleted RNA-seq |
| Low Throughput Method: | dHalo-mediated EWSR1::FLI1 degradation; CRISPR/Cas9-mediated EWSR1::FLI1 knockout; ChIP-qPCR; enhancer RNA RT-qPCR; NKX2-2 RT-qPCR |
About Target Gene
| Target gene : | NKX2-2 |
About TF
| TF name : | EWSR1::FLI1 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|
Enhancer Associated Network
Expression of Target Genes for the Enhancer