About Enhancer
| Enhancer ID: | E_03359 |
| Species: | Homo sapiens |
| Position : | chr9:27519197-27520919   |
| Year: | 2020 |
| Title: | A TGF-汕- and p63-Responsive Enhancer Regulates IFN-百 Expression in Human Keratinocytes. |
| Genome Build: | hg19 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 32060133 |
| Tissue: | skin of body |
| Tissue Ontology ID: | UBERON:0002097 |
| Cell Source: | Normal human keratinocytes |
| Cell Type: | keratinocyte |
| Cell Ontology ID: | CL:0000312 |
| Experiment Type: | Enhancer activity assay; Functional perturbation assay |
| High Throughput Method: | ChIP-seq; ENCODE epigenomic profiling |
| Low Throughput Method: | Luciferase reporter assay; EMSA; siRNA knockdown; ChIP-qPCR; qRT-PCR; Western blot |
About Target Gene
| Target gene : | IFNK |
About TF
| TF name : | SMAD4 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| SMAD4 | ALK1 signaling events | pid | 26 |
| SMAD4 | ALK2 signaling events | pid | 11 |
| SMAD4 | BMP receptor signaling | pid | 42 |
| SMAD4 | BMP signaling pathway ( TGF-beta_BMP Diagram(MolecularVariation) ) | inoh | 34 |
| SMAD4 | BMP Signalling Pathway | humancyc | 5 |
| SMAD4 | BMP2 signaling pathway(through Smad) ( TGF-beta_BMP Diagram(MolecularVariation) ) | inoh | 13 |
| SMAD4 | Downregulation of SMAD2/3:SMAD4 transcriptional activity | reactome | 23 |
| SMAD4 | Gene expression of smad6/7 by R-smad:smad4 ( TGF-beta_super_family_signaling_pathway(canonical) ) | inoh | 8 |
| SMAD4 | Gene expression of smad7 by R-smad:smad4 ( TGF-beta_super_family_signaling_pathway(canonical) ) | inoh | 7 |
| SMAD4 | HIF-1-alpha transcription factor network | pid | 67 |
| SMAD4 | LIF signaling pathway ( LIF signaling(JAK1 JAK2 STAT3) ) | inoh | 10 |
| SMAD4 | LKB1 signaling events | pid | 43 |
| SMAD4 | Negative regulation of (transcription by R-smad:smad4) in TGF beta super family signaling pathway ( TGF-beta_super_family_signaling_pathway(canonical) ) | inoh | 21 |
| SMAD4 | Regulation of cytoplasmic and nuclear SMAD2/3 signaling | pid | 20 |
| SMAD4 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| SMAD4 | Signaling by Activin | reactome | 11 |
| SMAD4 | Signaling by BMP | reactome | 23 |
| SMAD4 | Signaling by NODAL | reactome | 19 |
| SMAD4 | Signaling with Wnt (Canonical) ( C. elegans endoderm induction Wnt signaling pathway Diagram ) | inoh | 47 |
| SMAD4 | Signaling with Wnt (Canonical) ( Canonical Wnt signaling pathway Diagram ) | inoh | 47 |
| SMAD4 | Signaling with Wnt (Canonical) ( Drosophila Wingless/Wnt signaling pathway Diagram ) | inoh | 47 |
| SMAD4 | Signaling with Wnt (Canonical) ( Mammalian Wnt signaling pathway Diagram ) | inoh | 47 |
| SMAD4 | Signaling with Wnt (Canonical) ( Xenopus axis formation Wnt signaling pathway Diagram ) | inoh | 47 |
| SMAD4 | Signaling with Wnt (Mammal) ( Mammalian Wnt signaling pathway Diagram ) | inoh | 47 |
| SMAD4 | Signaling with Wnt (Xenopus) ( Xenopus axis formation Wnt signaling pathway Diagram ) | inoh | 19 |
| SMAD4 | SMAD2/3 MH2 Domain Mutants in Cancer | reactome | 7 |
| SMAD4 | SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription | reactome | 32 |
| SMAD4 | TGF-beta receptor signaling activates SMADs | reactome | 12 |
| SMAD4 | TGF-beta receptor signaling | pid | 53 |
| SMAD4 | TGF-beta signaling pathway | panther | 88 |
| SMAD4 | TGF-beta_super_family_signaling_pathway(canonical) ( TGF-beta_BMP Diagram(MolecularVariation) ) | inoh | 51 |
| SMAD4 | TGF_beta_Receptor | netpath | 220 |
| SMAD4 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| SMAD4 | Validated nuclear estrogen receptor alpha network | pid | 65 |
| SMAD4 | Validated targets of C-MYC transcriptional activation | pid | 80 |
| SMAD4 | Validated targets of C-MYC transcriptional repression | pid | 63 |
| SMAD4 | Wnt signaling pathway | panther | 250 |
| SMAD4 | Cell cycle | kegg | 124 |
| SMAD4 | Wnt signaling pathway | kegg | 135 |
| SMAD4 | TGF-beta signaling pathway | kegg | 82 |
| SMAD4 | Adherens junction | kegg | 70 |
| SMAD4 | Pathways in cancer | kegg | 321 |
| SMAD4 | Colorectal cancer | kegg | 63 |
| SMAD4 | Pancreatic cancer | kegg | 70 |
| SMAD4 | Chronic myeloid leukemia | kegg | 69 |
| SMAD4 | Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941 | wikipathways | 122 |
| SMAD4 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| SMAD4 | Hs_Heart_Development_WP1591_90186 | wikipathways | 28 |
| SMAD4 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
| SMAD4 | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
| SMAD4 | Hs_TGF-beta_Signaling_Pathway_WP366_90028 | wikipathways | 67 |
Enhancer Associated Network
Overlapping Enhancers (hg19)
5 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_03357 | chr9:27519197-27520919 | skin of body | keratinocyte | NA |
| E_03358 | chr9:27519197-27520919 | skin of body | keratinocyte | NA |
| E_03360 | chr9:27519197-27520919 | skin of body | keratinocyte | NA |
| E_03386 | chr9:27519749-27519900 | zone of skin | keratinocyte | NA |
| E_03387 | chr9:27520189-27520340 | zone of skin | keratinocyte | NA |
Expression of Target Genes for the Enhancer