About Enhancer
| Enhancer ID: | E_03578 |
| Species: | Mus musculus |
| Position : | chr1:13093655-13096290   |
| Year: | 2016 |
| Title: | NANOG alone induces germ cells in primed epiblast in vitro by activation of enhancers. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 26751055 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | D2 epiblast-like cells (+Dox Nanog) |
| Cell Type: | epiblast cell |
| Cell Ontology ID: | CL:0000352 |
| Experiment Type: | Enhancer activity assay; TF binding assay; Functional perturbation assay; Germline specification assay |
| High Throughput Method: | NANOG ChIP-seq; Expression microarray; H3K4me1/H3K27ac chromatin-data integration |
| Low Throughput Method: | Luciferase Reporter Assay; NANOG ChIP-qPCR; Doxycycline-inducible Nanog expression; RT-qPCR; PGCLC induction assay; Flow cytometry |
About Target Gene
| Target gene : | Prdm14 |
About TF
| TF name : | Nanog |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Nanog | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| Nanog | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| Nanog | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
6 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_01852 | chr1:13041306-13127367 | NA | NA | NA |
| E_01853 | chr1:13041306-13127367 | NA | NA | NA |
| E_01854 | chr1:13041306-13127367 | NA | NA | NA |
| E_01541 | chr1:13093655-13096290 | embryo | embryonic stem cell | NA |
| E_01560 | chr1:13093655-13096290 | embryo | primordial germ cell-like cell | NA |
| E_01561 | chr1:13093655-13096290 | embryo | epiblast-like cell | NA |
Expression of Target Genes for the Enhancer