About Enhancer
| Enhancer ID: | E_04148 |
| Species: | Mus musculus |
| Position : | chr7:142580000-142580293   |
| Year: | 2016 |
| Title: | Characterizing a distal muscle enhancer in the mouse Igf2 locus. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 26645089 |
| Tissue: | skeletal muscle tissue |
| Tissue Ontology ID: | UBERON:0001134 |
| Cell Source: | C3H10T1/2-derived myoblasts |
| Cell Type: | myoblast |
| Cell Ontology ID: | CL:0000056 |
| Experiment Type: | Enhancer activity assay; Functional perturbation assay; TF binding evidence; In vivo functional validation |
| High Throughput Method: | Published Myod1 occupancy data integration |
| Low Throughput Method: | BAC recombineering; 294-bp enhancer knockout; enhancer knock-in; stable BAC transfection; Ad-MyoD differentiation; RT-PCR; RT-qPCR; immunocytochemistry; DNA sequencing; In vivo phenotyping |
About Target Gene
| Target gene : | Igf2 |
About TF
| TF name : | Myod1 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Myod1 | C-MYB transcription factor network | pid | 87 |
| Myod1 | CDO in myogenesis | reactome | 29 |
| Myod1 | ID | netpath | 38 |
| Myod1 | Notch-mediated HES/HEY network | pid | 48 |
| Myod1 | Regulation of nuclear SMAD2/3 signaling | pid | 82 |
| Myod1 | Regulation of retinoblastoma protein | pid | 67 |
| Myod1 | Signaling events mediated by HDAC Class III | pid | 41 |
| Myod1 | Hs_ID_signaling_pathway_WP53_67360 | wikipathways | 8 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
No overlapping enhancers found in mm10 at this locus.
Expression of Target Genes for the Enhancer