About Enhancer
| Enhancer ID: | E_04337 |
| Species: | Mus musculus |
| Position : | chr6:122652222-122653558   |
| Year: | 2025 |
| Title: | Nucleosome fibre topology guides transcription factor binding to enhancers. |
| Genome Build: | mm9 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 39695228 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | Primary MEFs from 129 and 129/C57BL/6 E12.5-E13.5 embryos |
| Cell Type: | fibroblast |
| Cell Ontology ID: | CL:0000057 |
| Experiment Type: | TF binding assay; Enhancer activity assay; Chromatin accessibility assay; Nucleosome positioning assay; Chromatin topology assay; Cellular reprogramming assay |
| High Throughput Method: | ChIP-seq; ATAC-seq; RNA-seq; MNase-seq; Micro-C |
| Low Throughput Method: | PiggyBac dual fluorescent enhancer reporter assay; Flow cytometry; Fluorescence imaging; EMSA |
About Target Gene
| Target gene : | Nanog |
About TF
| TF name : | Sox2 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Sox2 | Deactivation of the beta-catenin transactivating complex | reactome | 42 |
| Sox2 | POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation | reactome | 13 |
| Sox2 | POU5F1 (OCT4), SOX2, NANOG repress genes related to differentiation | reactome | 10 |
| Sox2 | Transcriptional regulation of pluripotent stem cells | reactome | 28 |
| Sox2 | Hs_Endoderm_Differentiation_WP2853_88152 | wikipathways | 62 |
| Sox2 | Hs_Dopaminergic_Neurogenesis_WP2855_87239 | wikipathways | 19 |
| Sox2 | Hs_Wnt_Signaling_Pathway_and_Pluripotency_WP399_90291 | wikipathways | 12 |
| Sox2 | Hs_Mesodermal_Commitment_Pathway_WP2857_87780 | wikipathways | 47 |
| Sox2 | Hs_Ectoderm_Differentiation_WP2858_89329 | wikipathways | 56 |
Enhancer Associated Network
Overlapping Enhancers (mm9)
5 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_04335 | chr6:122652222-122653558 | embryo | fibroblast | NA |
| E_04336 | chr6:122652222-122653558 | embryo | fibroblast | NA |
| E_04343 | chr6:122652222-122653558 | embryo | embryonic stem cell | NA |
| E_04344 | chr6:122652222-122653558 | embryo | embryonic stem cell | NA |
| E_04345 | chr6:122652222-122653558 | embryo | embryonic stem cell | NA |
Expression of Target Genes for the Enhancer