About Enhancer
| Enhancer ID: | E_04535 |
| Species: | Mus musculus |
| Position : | chr8:83287756-83287955   |
| Year: | 2015 |
| Title: | PRDM16 enhances nuclear receptor-dependent transcription of the brown fat-specific Ucp1 gene through interactions with Mediator subunit MED1. |
| Genome Build: | mm10 |
| Enhancer Type: | Enhancer |
| Condition: | Normal |
| PMID: | 25644605 |
| Tissue: | embryo |
| Tissue Ontology ID: | UBERON:0000922 |
| Cell Source: | Mouse Embryonic Fibroblasts |
| Cell Type: | fibroblast |
| Cell Ontology ID: | CL:0000057 |
| Experiment Type: | Enhancer recruitment assay; TF/cofactor binding assay; Functional perturbation assay; In vitro transcription assay |
| Low Throughput Method: | PRDM16 ChIP-qPCR; immobilized enhancer-template recruitment assay; in vitro transcription assay; co-immunoprecipitation; GST pull-down; MED1 knockdown; thyroid hormone receptor perturbation; forskolin treatment; RT-qPCR; Western blot |
About Target Gene
| Target gene : | Ucp1 |
About TF
| TF name : | Prdm16 |
About Regulatory State
| Regulatory State : | Active |
Upstream Pathway Annotation of TF
| GeneName | Pathway Name | Source | Gene Number |
|---|---|---|---|
| Prdm16 | PKMTs methylate histone lysines | reactome | 64 |
Enhancer Associated Network
Overlapping Enhancers (mm10)
1 found
| Enhancer ID | Position | Tissue | Cell | Disease |
|---|---|---|---|---|
| E_04560 | chr8:83287848-83288068 | blood / hematopoietic system | NA | NA |
Expression of Target Genes for the Enhancer